Introduction：Basic information about CAS 347174-05-4|Ferrostatin-1, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Ferrostatin-1 |
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| CAS Number | 347174-05-4 | Molecular Weight | 262.347 |
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| Density | 1.1±0.1 g/cm3 | Boiling Point | 437.3±35.0 °C at 760 mmHg |
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| Molecular Formula | C15H22N2O2 | Melting Point | / |
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| MSDS | ChineseUSA | Flash Point | 218.3±25.9 °C |
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| Symbol |
GHS07 | Signal Word | Warning |
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Names
| Name | Ethyl 3-amino-4-(cyclohexylamino)benzoate |
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| Synonym | More Synonyms |
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Ferrostatin-1 BiologicalActivity
| Description | Ferrostatin-1 is a potent inhibitor of ferroptosis with an EC50 of 60 nM. |
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| Related Catalog | Signaling Pathways >>Apoptosis >>FerroptosisResearch Areas >>Cancer |
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| Target | EC50: 60 nM (Ferroptosis)[1] |
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| In Vitro | Ferrostatin-1 is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1 does not inhibit ERK phosphorylation or arrest the proliferation of HT-1080 cells. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS[1]. Cells pretreated with 0.4 μM Ferrostatin-1 displays significantly reduce intracellular reactive oxygen species (ROS) and nitrogen species (RNS) below basal levels. Additionally, increased intracellular ROS levels are also significantly lowered below basal levels by a 0.4 µM Ferrostatin-1 pretreatment. Ferrostatin-1 treatment for 24 h does not change the expression level of i-NOS in SHSY-5Y cell when compare with vehicle (0.02 % DMSO) treated cells[2]. |
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| Kinase Assay | The cells treated for 24 h with Ferrostatin-1 are washed three times with phosphate-buffered saline (PBS) and pelleted by centrifugation. The supernatant is removed and 80 μL of lysis buffer is added to the cells and then stored overnight at -20°C. Subsequently, the cells are centrifuged at 10,000 RPM for 12 min and both the pellet and supernatant are stored for future use[2]. |
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| Cell Assay | SH-S5Y cells are seeded in 24-well plates. After overnight incubation, the cells are exposed for 24 h to 1 µM of Ferrostatin-1, under standard growth conditions. Bright field photomicrographs are captured using an inverted microscope equipped with 10× objective in a live-cell modality[2]. |
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| References | [1]. Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72. [2]. Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58. |
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Chemical & Physical Properties
| Density | 1.1±0.1 g/cm3 |
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| Boiling Point | 437.3±35.0 °C at 760 mmHg |
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| Molecular Formula | C15H22N2O2 |
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| Molecular Weight | 262.347 |
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| Flash Point | 218.3±25.9 °C |
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| Exact Mass | 262.168121 |
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| PSA | 64.35000 |
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| LogP | 3.90 |
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| Appearance of Characters | , faint purple to brown |
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| Vapour Pressure | 0.0±1.1 mmHg at 25°C |
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| Index of Refraction | 1.595 |
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| InChIKey | UJHBVMHOBZBWMX-UHFFFAOYSA-N |
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| SMILES | CCOC(=O)c1ccc(NC2CCCCC2)c(N)c1 |
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| Storage condition | 2-8°C |
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| Water Solubility | DMSO: soluble10mg/mL, clear |
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Safety Information
| Symbol |
GHS07 |
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| Signal Word | Warning |
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| Hazard Statements | H315-H319-H335 |
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| Precautionary Statements | P261-P305 + P351 + P338 |
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| Hazard Codes | Xi |
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| Risk Phrases | 36/37/38 |
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| Safety Phrases | 26 |
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| RIDADR | NONH for all modes of transport |
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| HS Code | 2922499990 |
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Customs
| HS Code | 2922499990 |
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| Summary | HS:2922499990 other amino-acids, other than those containing more than one kind of oxygen function, and their esters; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward) MFN tariff:6.5% General tariff:30.0% |
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Synonyms
| Ethyl 3-amino-4-(cyclohexylamino)benzoate |
| 3-Amino-4-cyclohexylaminobenzoic acid ethyl ester |
| Benzoic acid, 3-amino-4-(cyclohexylamino)-, ethyl ester |
| ethyl 3-amino-4-cyano-1-phenyl-1H-pyrrole-2-carboxylate |
| Ferrostatin-1 (Fer-1) |
| 3-amino-4-cyan-1-phenyl-pyrrol-2-carbonsaeureethylester |
| ethyl 3-amino-4-cyclohexylaminobenzoate |
| Ferrostatin-1 |
| Benzoic acid (3-amino-4-(cyclohexylamino) |
| 3-Amino-4-cyclohexylamino benzoic acid ethyl ester |
