Introduction：Basic information about CAS 550-82-3|3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | 3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide |
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| CAS Number | 550-82-3 | Molecular Weight | 251.17000 |
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| Density | 1.57g/cm3 | Boiling Point | 477.2ºC at 760mmHg |
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| Molecular Formula | C12H6NNaO4 | Melting Point | / |
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| MSDS | / | Flash Point | 242.4ºC |
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Names
| Name | resazurin |
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| Synonym | More Synonyms |
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3H-Phenoxazin-3-one,7-hydroxy-, 10-oxide BiologicalActivity
| Description | Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)[1][2]. |
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| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
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| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Cell viability[1][2]: 1. Defrost the Resazurin solution in a 37°C water bath. 2. Place the cells on a 96-well plate, and washed by PBS (avoid light). 3. Remove the PBS wash, then add 500 μL (1µg/mL) of Resazurin solution. 4. Place the plate, in the incubator for 30 min (incubation time depends on cell type and cell number). 5. Measure Resorufin fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader. |
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| References | [1]. Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15. [2]. Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60. |
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Chemical & Physical Properties
| Density | 1.57g/cm3 |
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| Boiling Point | 477.2ºC at 760mmHg |
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| Molecular Formula | C12H6NNaO4 |
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| Molecular Weight | 251.17000 |
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| Flash Point | 242.4ºC |
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| Exact Mass | 251.01900 |
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| PSA | 78.73000 |
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| LogP | 2.47010 |
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| Index of Refraction | 1.74 |
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| InChIKey | PLXBWHJQWKZRKG-UHFFFAOYSA-N |
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| SMILES | O=c1ccc2[n+]([O-])c3ccc(O)cc3oc-2c1 |
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Toxicological Information
CHEMICAL IDENTIFICATION - RTECS NUMBER :
- SP7700000
- CHEMICAL NAME :
- 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide
- CAS REGISTRY NUMBER :
- 550-82-3
- BEILSTEIN REFERENCE NO. :
- 0221382
- LAST UPDATED :
- 199701
- DATA ITEMS CITED :
- 4
- MOLECULAR FORMULA :
- C12-H7-N-O4
- MOLECULAR WEIGHT :
- 229.20
- WISWESSER LINE NOTATION :
- T C666 BN FV IOJ BO LQ
HEALTH HAZARD DATAACUTE TOXICITY DATA - TYPE OF TEST :
- LDLo - Lowest published lethal dose
- ROUTE OF EXPOSURE :
- Oral
- SPECIES OBSERVED :
- Rodent - rat
- DOSE/DURATION :
- 500 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
- REFERENCE :
- NCNSA6 National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. (Washington, DC) Volume(issue)/page/year: 5,24,1953
- TYPE OF TEST :
- LDLo - Lowest published lethal dose
- ROUTE OF EXPOSURE :
- Intravenous
- SPECIES OBSERVED :
- Rodent - mouse
- DOSE/DURATION :
- 179 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
- REFERENCE :
- TXAPA9 Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 44,225,1978 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 84815 No. of Facilities: 303 (estimated) No. of Industries: 1 No. of Occupations: 7 No. of Employees: 3485 (estimated) No. of Female Employees: 2576 (estimated)
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Safety Information
Customs
| HS Code | 2934999090 |
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| Summary | 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
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Synonyms
| 3H-Phenoxazin-3-one,7-hydroxy-,10-oxide |
| azoresorcin |
| 7-Hydroxy-10-oxy-phenoxazin-3-on |
| Diazoresorcinal |
| 7-hydroxy-3h-fenoxazin-3-on-10-oxid |
| 7-hydroxy-3h-phenoxazin-3-on10-oxide |
| PHENOXYAZIN-3-ONE,7-HYDROXY-,10-OXIDE |
| Resazurin tablets |
| 7-HYDROXY-10-OXY-PHENOXAZIN-3-ONE |
| 7-hydroxy-3h-phenoxazin-3-one10-oxide |
