Introduction：Basic information about CAS 65189-71-1|Kemptide, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Kemptide |
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| CAS Number | 65189-71-1 | Molecular Weight | 771.908 |
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| Density | 1.4±0.1 g/cm3 | Boiling Point | / |
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| Molecular Formula | C32H61N13O9 | Melting Point | / |
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| MSDS | / | Flash Point | / |
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Names
| Name | Kemptide acetate salt |
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| Synonym | More Synonyms |
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Kemptide BiologicalActivity
| Description | Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA). |
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| Related Catalog | Signaling Pathways >>Protein Tyrosine Kinase/RTK >>PKASignaling Pathways >>Stem Cell/Wnt >>PKAResearch Areas >>Metabolic DiseasePeptides |
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| In Vitro | Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase[2]. |
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| Kinase Assay | PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP[2]. |
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| References | [1]. Kübler D, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55. [2]. Duncan FE, et al. Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation. Biol Reprod. 2014 Mar 20;90(3):63. |
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Chemical & Physical Properties
| Density | 1.4±0.1 g/cm3 |
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| Molecular Formula | C32H61N13O9 |
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| Molecular Weight | 771.908 |
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| Exact Mass | 771.471497 |
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| PSA | 381.95000 |
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| LogP | -1.39 |
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| Index of Refraction | 1.626 |
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| InChIKey | WIGDGIGALMYEBW-UHFFFAOYSA-N |
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| SMILES | CC(C)CC(N)C(=O)NC(CCCN=C(N)N)C(=O)NC(CCCN=C(N)N)C(=O)NC(C)C(=O)NC(CO)C(=O)NC(CC(C)C)C(=O)NCC(=O)O |
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| Storage condition | -20°C |
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Safety Information
| Hazard Codes | Xn |
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| Safety Phrases | S22-S24/25 |
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| WGK Germany | 3.0 |
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Synonyms
| L-leucyl-N-(diaminomethylidene)-L-ornithyl-N-(diaminomethylidene)-L-ornithyl-L-alanyl-L-seryl-L-leucylglycine |
| kemptide |
| Glycine, N-(N-(N-(N-(N2-(N2-L-leucyl-L-arginyl)-L-arginyl)-L-alanyl)-L-seryl)-L-leucyl)- |
| Glycine, L-leucyl-N-(diaminomethylene)-L-ornithyl-N-(diaminomethylene)-L-ornithyl-L-alanyl-L-seryl-L-leucyl- |
| L-Leucyl-N-(diaminomethylene)-L-ornithyl-N-(diaminomethylene)-L-ornithyl-L-alanyl-L-seryl-L-leucylglycine |
| MFCD00076710 |
| LRRASLG |
