CAS 94367-21-2|Suc-Leu-Leu-Val-Tyr-AMC
| Common Name | Suc-Leu-Leu-Val-Tyr-AMC | ||
|---|---|---|---|
| CAS Number | 94367-21-2 | Molecular Weight | 763.87600 |
| Density | 1.249g/cm3 | Boiling Point | 1116.8ºC at 760mmHg |
| Molecular Formula | C40H53N5O10 | Melting Point | / |
| MSDS | USA | Flash Point | 629.2ºC |
Names
| Name | succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin |
|---|---|
| Synonym | More Synonyms |
Suc-Leu-Leu-Val-Tyr-AMC BiologicalActivity
| Description | Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. Sequence: Suc-Leu-Leu-Val-Tyr. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsPeptidesResearch Areas >>Others |
| In Vitro | Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effectof TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2]. |
| Kinase Assay | Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers[3]. |
| References | [1]. Roberta L. Debiasi, et al. Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors. J Virol. 1999 Jan; 73(1): 695–701. [2]. Tadlock L, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85. Epub 2003 Mar 19. [3]. Gardner RC, et al. Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells. Biochem J. 2000 Mar, 2:447-54. |
Chemical & Physical Properties
| Density | 1.249g/cm3 |
|---|---|
| Boiling Point | 1116.8ºC at 760mmHg |
| Molecular Formula | C40H53N5O10 |
| Molecular Weight | 763.87600 |
| Flash Point | 629.2ºC |
| Exact Mass | 763.37900 |
| PSA | 233.24000 |
| LogP | 5.17700 |
| Index of Refraction | 1.577 |
| InChIKey | UVFAEQZFLBGVRM-MSMWPWNWSA-N |
| SMILES | Cc1cc(=O)oc2cc(NC(=O)C(Cc3ccc(O)cc3)NC(=O)C(NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)CCC(=O)O)C(C)C)ccc12 |
| Storage condition | −20°C |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
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Synonyms
| z-llvy-amc |
| suc-leu-leu-val-tyr-amc |
| suc-llvy-amc |
| suc-leu-leu-val-tyr-7-amino-4-methylcoumarin |
| chymotrypsin substrate iii,fluorogenic |
| n-succinyl-leu-leu-val-tyr 7-amido-4-methylcoumarin |
| MFCD00080248 |
| suc-leu-leu-val-tyr-mca |
