CAS 3482-57-3|4-Nitrophenyl β-D-Cellobioside
| Common Name | 4-Nitrophenyl β-D-Cellobioside | ||
|---|---|---|---|
| CAS Number | 3482-57-3 | Molecular Weight | 463.39000 |
| Density | 1.7 g/cm3 | Boiling Point | 795.6ºC at 760 mmHg |
| Molecular Formula | C18H25NO13 | Melting Point | 249-250ºC |
| MSDS | ChineseUSA | Flash Point | 435ºC |
Names
| Name | 4-Nitrophenyl β-D-cellobioside |
|---|---|
| Synonym | More Synonyms |
4-Nitrophenyl β-D-Cellobioside BiologicalActivity
| Description | 4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2]. |
|---|---|
| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
| References | [1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53. [2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31. |
Chemical & Physical Properties
| Density | 1.7 g/cm3 |
|---|---|
| Boiling Point | 795.6ºC at 760 mmHg |
| Melting Point | 249-250ºC |
| Molecular Formula | C18H25NO13 |
| Molecular Weight | 463.39000 |
| Flash Point | 435ºC |
| Exact Mass | 463.13300 |
| PSA | 224.35000 |
| Index of Refraction | 1.67 |
| InChIKey | IAYJZWFYUSNIPN-KFRZSCGFSA-N |
| SMILES | O=[N+]([O-])c1ccc(OC2OC(CO)C(OC3OC(CO)C(O)C(O)C3O)C(O)C2O)cc1 |
| Storage condition | 2-8°C |
| Water Solubility | methanol: water (2:3): 50 mg/mL, clear, faintly yellow |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Safety Phrases | S24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 29400090 |
Articles25
More Articles| Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2. Biochemistry 38(30) , 9746-51, (1999) Endocellulase E2 from the thermophilic bacterium Thermomonospora fusca is a member of glycosyl-hydrolase family 6 and is active from pH 4 to 10. Enzymes in this family hydrolyze beta-1,4-glycosidic bo... | |
| Structural changes of cellobiohydrolase I (1,4-beta-D-glucan-cellobiohydrolase I, CBHI) and PNPC (p-nitrophenyl-beta-D-cellobioside) during the binding process. Sci. China,. C, Life Sci. 51(5) , 459-69, (2008) Conformational changes to 1,4-beta-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl beta-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrom... | |
| The competitive inhibition of Trichoderma reesei C30 cellobiohydrolase I by guanidine hydrochloride. FEBS Lett. 270(1-2) , 143-6, (1990) The p-nitrophenylcellobiosidase (PNPCase) activity of Trichoderma reesei cellobiohydrolase I (CBH I) was competitively inhibited by concentrations of guanidine hydrochloride (Gdn HCl) that did not aff... |
Synonyms
| 4-NITROPHENYL-β-D-CELLOBIOSIDE |
| 4-Nitrophenyl β-D-Cellobioside |
| 4-NITROPHENYL-BETA-D-CELLOBIOSIDE |
| 4-Nitrophenyl beta-D-Cellobioside |
| MFCD00069845 |
