CAS 479-50-5|Lucanthone

Introduction：Basic information about CAS 479-50-5|Lucanthone, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Lucanthone BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Synonyms

Common Name	Lucanthone
CAS Number	479-50-5	Molecular Weight	340.48200
Density	1.184g/cm3	Boiling Point	512.4ºC at 760 mmHg
Molecular Formula	C₂₀H₂₄N₂OS	Melting Point	195-196ºC
MSDS	/	Flash Point	263.7ºC

Names

Name	lucanthone
Synonym	More Synonyms

Lucanthone BiologicalActivity

Description	Lucanthone is an endonuclease inhibitor of Apurinic endonuclease-1 (APE-1).
Related Catalog	Signaling Pathways >>Autophagy >>AutophagyResearch Areas >>Cancer
Target	APE-1[1]
In Vitro	Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. To investigate the anticancer activity of Lucanthone, cell viability is measured by MTT assay. Lucanthone reduces cell viability to a similar extent in a panel of seven breast cancer cell lines. In addition, a direct comparison reveals that Lucanthone is significantly more potent than Chloroquine (CQ) at reducing breast cancer cell viability with a mean IC50 of 7.2 μM versus 66 μM for CQ. Measurement of cell viability in two representative cell lines (MDA-MB-231 and BT-20) by ATPlite assay and trypan blue exclusion reveals comparable results[2].
Cell Assay	Cell viability is assessed by MTT assay. Cells are seeded into 96-well microculture plates at 10,000 cells per well and allowed to attach for 24 h. Cells are then treated with Lucanthone (0, 0.5, 1, 5, 10, 20 and 40 μM), Chloroquine, Vorinostat, or combinations for 72 h. Following drug treatment, MTT is added and cell viability is quantified using a BioTek microplate reader. Effects on cell viability are also determined by measuring ATP levels using the ATPlite assay system and by trypan blue exclusion. Pro-apoptotic effects following in vitro drug exposure are quantified by propidium iodide (PI) staining and fluorescence-activated cell sorting (FACS) analysis of sub-G0/G1 DNA content[2].
References	[1]. Chowdhury SM, et al. Graphene nanoribbons as a drug delivery agent for lucanthone mediated therapy of glioblastoma multiforme. Nanomedicine. 2015 Jan;11(1):109-18. [2]. Carew JS, et al. Lucanthone is a novel inhibitor of autophagy that induces cathepsin D-mediated apoptosis. J Biol Chem. 2011 Feb 25;286(8):6602-13.

Chemical & Physical Properties

Density	1.184g/cm3
Boiling Point	512.4ºC at 760 mmHg
Melting Point	195-196ºC
Molecular Formula	C₂₀H₂₄N₂OS
Molecular Weight	340.48200
Flash Point	263.7ºC
Exact Mass	340.16100
PSA	60.58000
LogP	4.54980
Vapour Pressure	1.3E-10mmHg at 25°C
Index of Refraction	1.638
InChIKey	FBQPGGIHOFZRGH-UHFFFAOYSA-N
SMILES	CCN(CC)CCNc1ccc(C)c2sc3ccccc3c(=O)c12
Storage condition	2-8℃

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: XO1605500

CHEMICAL NAME :: Thioxanthen-9-one, 1-((2-(diethylamino)ethyl)amino)-4-methyl-

CAS REGISTRY NUMBER :: 479-50-5

BEILSTEIN REFERENCE NO. :: 0312369

LAST UPDATED :: 199707

DATA ITEMS CITED :: 26

MOLECULAR FORMULA :: C20-H24-N2-O-S

MOLECULAR WEIGHT :: 340.52

WISWESSER LINE NOTATION :: T C666 BS IVJ D1 GM2N2&2

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 56 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intramuscular

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 400 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: DNA inhibition

TYPE OF TEST :: Mutation test systems - not otherwise specified

MUTATION DATA

TYPE OF TEST :: DNA adduct

TEST SYSTEM :: Mammal - species unspecified Lymphocyte

DOSE/DURATION :: 6 umol/L

REFERENCE :: PMSBA4 Progress in Molecular and Subcellular Biology. (Springer-Verlag, Heidelberger Pl. 3, D-1000 Berlin 33, Fed. Rep. Ger.) V.1- 1969- Volume(issue)/page/year: 2,232,1971

Synonyms

1-(2-Diethylamino-ethylamino)-4-methylthiaxanthon,Lucanthon

Lycanthone

thioxanthone

1-(2-diethylamino-ethylamino)-4-methyl-thioxanthen-9-one

1-<2-Diaethylamino-aethylamino>-4-methyl-thioxanthenon-(9)

1-[[2-(Diethylamino)ethyl]amino]-4-methyl-9H-thioxanthen-9-one

1-(2-Diaethylamino-aethylamino)-4-methyl-thioxanthen-9-on

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