CAS 333338-18-4|4-Nitrophenyl phosphate disodium hexahydrate
| Common Name | 4-Nitrophenyl phosphate disodium hexahydrate | ||
|---|---|---|---|
| CAS Number | 333338-18-4 | Molecular Weight | 371.14400 |
| Density | / | Boiling Point | 457.8ºC at 760mmHg |
| Molecular Formula | C6H16NNa2O12P | Melting Point | >300ºC(lit.) |
| MSDS | ChineseUSA | Flash Point | 230.7ºC |
Names
| Name | disodium,(4-nitrophenyl) phosphate,hexahydrate |
|---|---|
| Synonym | More Synonyms |
BiologicalActivity
| Description | 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1]. |
|---|---|
| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
| In Vitro | 4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2]. |
| References | [1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205. [2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877. |
Chemical & Physical Properties
| Boiling Point | 457.8ºC at 760mmHg |
|---|---|
| Melting Point | >300ºC(lit.) |
| Molecular Formula | C6H16NNa2O12P |
| Molecular Weight | 371.14400 |
| Flash Point | 230.7ºC |
| Exact Mass | 371.02100 |
| PSA | 183.43000 |
| LogP | 2.08010 |
| Appearance of Characters | tablets | white to pale yellow |
| Storage condition | 2-8°C |
| Water Solubility | H2O: 25 mg/mL at 20 °C, clear, yellow-green |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Safety Phrases | 24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
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Synonyms
| MFCD00066288 |
| di-Sodium 4-nitrophenyl phosphate |
| pNPP disodium salt hexahydrate |
| DisodiuM 4-Nitrophenylphosphate Hexahydrate |
| Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt Hexahydrate |
| Phosphatase substrate |
| 4-Nitrophenyl Phosphate,Disodium Salt Hexahydrate |
| p-Nitrophenyl phosphate disodium hexahydrate |
| pNPP disodium hexahydrate |
| 4-Nitrophenyl phosphate disodium salt hexahydrate |
| p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di |
| Sodium 4-nitrophenyl phosphate hexahydrate |
| EINECS 224-246-5 |
| Disodium 4-Nitrophenyl Phosphate Hexahydrate |
