Introduction：Basic information about CAS 107015-83-8|Amyloid β-Protein (12-28), including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Amyloid β-Protein (12-28) |
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| CAS Number | 107015-83-8 | Molecular Weight | 1955.18000 |
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| Density | / | Boiling Point | / |
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| Molecular Formula | C89H135N25O25 | Melting Point | / |
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| MSDS | / | Flash Point | / |
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Names
| Name | Amyloid β-Peptide (12-28) (human) |
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| Synonym | More Synonyms |
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Amyloid β-Protein (12-28) BiologicalActivity
| Description | β-amyloid (12-28) is a 17-aa peptide fragment, which can produce amyloid aggregates, used in the research of Alzheimer’sdisease. |
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| Related Catalog | Signaling Pathways >>Neuronal Signaling >>Amyloid-βResearch Areas >>Neurological DiseasePeptides |
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| Target | Amyloid-β[1] |
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| In Vitro | β-amyloid (12-28) preared freshly in solution has no cytotoxicity, but aged β12-28 peptide (0.001-40 μM) reduces the viability of PC12 cells in a concentration dependent manner[1]. |
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| Cell Assay | PC12 pheochromocytoma cell lines are routinely cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum,5% horse serum, 1% glutamine and 1% penicillin/streptomycin. Exponentially growing cellsare plated at 30000 cells per well per 100 µL of fresh medium in 96-well tissue cultures plates. Toxicity assays are carried out by measurementof MTT reduction. Two days after plating PC12 pheochromocytoma cell lines are treated with different concentrations of aged β-amyloid (12-28). After 22 h, MTT (5 mg/mL) wasadded and incubation is continued for a further2 h. Cells are lysed and after 24 h at 37°C,colorimetric determination of MTT reduction is made at 550 nm. Positive controls consist of cells in culture medium. Assay values obtained with these controls are taken as 100% viability[1]. |
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| References | [1]. Rabanal F, et al. Structural, kinetic and cytotoxicity aspects of 12-28 beta-amyloid protein fragment: a reappraisal. J Pept Sci. 2002 Oct;8(10):578-88. |
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Chemical & Physical Properties
| Molecular Formula | C89H135N25O25 |
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| Molecular Weight | 1955.18000 |
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| Exact Mass | 1954.01000 |
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| PSA | 819.33000 |
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| LogP | 2.72410 |
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| InChIKey | WOMPQPMHRDDZMP-IJBWYZRXSA-N |
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| SMILES | CC(C)CC(NC(=O)C(CCCCN)NC(=O)C(CCC(N)=O)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(N)C(C)C)C(=O)NC(C(=O)NC(Cc1ccccc1)C(=O)NC(Cc1ccccc1)C(=O)NC(C)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(C(=O)NCC(=O)NC(CO)C(=O)NC(CC(N)=O)C(=O)NC(CCCCN)C(=O)O)C(C)C)C(C)C |
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| Storage condition | −20°C |
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Safety Information
Synonyms
| MFCD00133075 |
| vhhqklvffaedvgsnk |
| sp17 |
| amyloid b-protein fragment 12-28 |
| m.w. 1955.20 c89h135n25o25 |
| β-amyloid (12-28) |
