CAS 475-25-2|Hematein

Introduction：Basic information about CAS 475-25-2|Hematein, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Hematein BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Safety Information
6. Articles24
7. Synonyms

Common Name	Hematein
CAS Number	475-25-2	Molecular Weight	300.26300
Density	1.77g/cm3	Boiling Point	753.1ºC at 760mmHg
Molecular Formula	C₁₆H₁₂O₆	Melting Point	180ºC
MSDS	USA	Flash Point	289ºC

Names

Name	3,4,6a,10-tetrahydroxy-6,7-dihydroindeno[2,1-c]chromen-9-one
Synonym	More Synonyms

Hematein BiologicalActivity

Description	Hematein is a oxidation product of hematoxylin acted as a dye[1]. Hematein is an allosteric casein kinase II inhibitor with an IC50 of 0.74 μM. Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway and increases apoptosis in lung cancer cells[2].
Related Catalog	Signaling Pathways >>Apoptosis >>ApoptosisResearch Areas >>CancerSignaling Pathways >>Stem Cell/Wnt >>WntSignaling Pathways >>PI3K/Akt/mTOR >>Akt
Target	CKII:0.74 μM (IC50) Akt Wnt
In Vitro	Hematein (10-100 μM; 14 days) inhibits cells growth in A427 lung cancer cells[1]. Hematein (50 and 100 μM; 48 hours) induces apoptosis in A427 lung cancer cells[1]. Hematein (50 and 100 μM; 48 hours) inhibits CK2-specific Akt phosphorylation[1]. Cell Viability Assay[1] Cell Line: A427 (HTB-53) cell line Concentration: 10-100 μM Incubation Time: 14 days Result: Inhibited cells growth. Apoptosis Analysis[1] Cell Line: A427 (HTB-53) cell line Concentration: 50 and 100 μM Incubation Time: 48 hours Result: Induced apoptosis in A427 lung cancer cells. Western Blot Analysis[1] Cell Line: A427 (HTB-53) cell line Concentration: 50 and 100 μM Incubation Time: 48 hours Result: Inhibited CK2-specific Akt phosphorylation.
In Vivo	Hematein (intraperitoneal injections; 50 mg/kg; twice a week for 6 weeks) inhibits tumor growth in A427 lung cancer cell xenografts[1]. Animal Model: Female athymic BALB/c nude mice (6-week-old) [1] Dosage: 50 mg/kg Administration: Intraperitoneal injections; twice a week for 6 weeks Result: Inhibited tumor growth in A427 lung cancer cell xenografts.
References	[1]. Shirai, K., & Matsuoka, M. (1996). Structure and properties of hematein derivatives. Dyes and Pigments, 32(3), 159–169. [2]. Hung MS, et al. Hematein, a casein kinase II inhibitor, inhibits lung cancer tumor growth in a murine xenograftmodel.Int J Oncol. 2013 Nov;43(5):1517-22.

Chemical & Physical Properties

Density	1.77g/cm3
Boiling Point	753.1ºC at 760mmHg
Melting Point	180ºC
Molecular Formula	C₁₆H₁₂O₆
Molecular Weight	300.26300
Flash Point	289ºC
Exact Mass	300.06300
PSA	107.22000
LogP	1.32960
Vapour Pressure	1.32E-17mmHg at 25°C
Index of Refraction	1.765

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DE3120000

CHEMICAL NAME :: Benz(b)indeno(1,2-d)pyran-9(6H)-one, 6a,7-dihydro-3,4,6a,10-tetrahydroxy-

CAS REGISTRY NUMBER :: 475-25-2

BEILSTEIN REFERENCE NO. :: 0093039

LAST UPDATED :: 199701

DATA ITEMS CITED :: 3

MOLECULAR FORMULA :: C16-H12-O6

MOLECULAR WEIGHT :: 300.28

WISWESSER LINE NOTATION :: T C6 B566 FV LO GH&TT&J EQ JQ NQ OQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Rodent - hamster Ovary

DOSE/DURATION :: 20 umol/L/5H (Continuous)

REFERENCE :: ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 1,27,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X6653 No. of Facilities: 25 (estimated) No. of Industries: 1 No. of Occupations: 2 No. of Employees: 760 (estimated) No. of Female Employees: 615 (estimated)

Safety Information

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
Safety Phrases	S24/25
RIDADR	NONH for all modes of transport

Articles24

The CAP1/Prss8 catalytic triad is not involved in PAR2 activation and protease nexin-1 (PN-1) inhibition.

FASEB J. 28(11) , 4792-805, (2014)

Serine proteases, serine protease inhibitors, and protease-activated receptors (PARs) are responsible for several human skin disorders characterized by impaired epidermal permeability barrier function...

Necrosis- and apoptosis-related Met cleavages have divergent functional consequences.

Cell Death Dis. 6 , e1769, (2015)

Upon activation by its ligand hepatocyte growth factor/scatter factor, the receptor tyrosine kinase Met promotes survival, proliferation, and migration of epithelial cells during embryogenesis. Deregu...

The Effects of a Selective CK2 Inhibitor on Anti-glomerular Basement Membrane Glomerulonephritis in Rats.

Biol. Pharm. Bull. 38 , 1345-51, (2015)

Protein kinase CK2 ("casein kinase II") is a protein serine/threonine kinase that plays critical roles in biological processes such as cell growth, cell cycle progression, and apoptosis. So far, we ha...

Synonyms

hydroxybrazilein

Haematein

EINECS 207-492-8

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