CAS 2321-07-5|Fluorescein
Introduction：Basic information about CAS 2321-07-5|Fluorescein, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Fluorescein | ||
|---|---|---|---|
| CAS Number | 2321-07-5 | Molecular Weight | 332.306 |
| Density | 1.6±0.1 g/cm3 | Boiling Point | 620.8±55.0 °C at 760 mmHg |
| Molecular Formula | C20H12O5 | Melting Point | 320 °C(lit.) |
| MSDS | ChineseUSA | Flash Point | 232.6±25.0 °C |
| Symbol | GHS07 | Signal Word | Warning |
Names
| Name | fluorescein |
|---|---|
| Synonym | More Synonyms |
Fluorescein BiologicalActivity
| Description | Fluorescein is a synthetic fluorescent tracer. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent[1]. |
| References | [1]. R Sjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
Chemical & Physical Properties
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 620.8±55.0 °C at 760 mmHg |
| Melting Point | 320 °C(lit.) |
| Molecular Formula | C20H12O5 |
| Molecular Weight | 332.306 |
| Flash Point | 232.6±25.0 °C |
| Exact Mass | 332.068481 |
| PSA | 87.74000 |
| LogP | 2.98 |
| Vapour Pressure | 0.0±1.9 mmHg at 25°C |
| Index of Refraction | 1.792 |
| InChIKey | GNBHRKFJIUUOQI-UHFFFAOYSA-N |
| SMILES | O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccccc21 |
| Storage condition | −20°C |
| Water Solubility | insoluble |
Toxicological Information
CHEMICAL IDENTIFICATION |
ACUTE TOXICITY DATA - TYPE OF TEST :
- LDLo - Lowest published lethal dose
- ROUTE OF EXPOSURE :
- Intraperitoneal
- SPECIES OBSERVED :
- Rodent - rat
- DOSE/DURATION :
- 600 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
- TYPE OF TEST :
- LD50 - Lethal dose, 50 percent kill
- ROUTE OF EXPOSURE :
- Intravenous
- SPECIES OBSERVED :
- Rodent - mouse
- DOSE/DURATION :
- 300 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
- TYPE OF TEST :
- LDLo - Lowest published lethal dose
- ROUTE OF EXPOSURE :
- Unreported
- SPECIES OBSERVED :
- Rodent - mouse
- DOSE/DURATION :
- 600 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
- TYPE OF TEST :
- LDLo - Lowest published lethal dose
- ROUTE OF EXPOSURE :
- Intravenous
- SPECIES OBSERVED :
- Rodent - rabbit
- DOSE/DURATION :
- 300 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
- TYPE OF TEST :
- LDLo - Lowest published lethal dose
- ROUTE OF EXPOSURE :
- Unreported
- SPECIES OBSERVED :
- Rodent - guinea pig
- DOSE/DURATION :
- 400 mg/kg
- TOXIC EFFECTS :
- Details of toxic effects not reported other than lethal dose value
MUTATION DATA - TYPE OF TEST :
- DNA adduct
- TEST SYSTEM :
- Bacteria - Escherichia coli
- DOSE/DURATION :
- 15 umol/L
- REFERENCE :
- MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 33350 No. of Facilities: 495 (estimated) No. of Industries: 18 No. of Occupations: 21 No. of Employees: 5188 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 33350 No. of Facilities: 2171 (estimated) No. of Industries: 42 No. of Occupations: 55 No. of Employees: 88495 (estimated) No. of Female Employees: 21558 (estimated)
- TYPE OF TEST :
- DNA adduct
- TEST SYSTEM :
- Bacteria - Escherichia coli
- DOSE/DURATION :
- 15 umol/L
- REFERENCE :
- MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 33350 No. of Facilities: 495 (estimated) No. of Industries: 18 No. of Occupations: 21 No. of Employees: 5188 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 33350 No. of Facilities: 2171 (estimated) No. of Industries: 42 No. of Occupations: 55 No. of Employees: 88495 (estimated) No. of Female Employees: 21558 (estimated)
Safety Information
| Symbol | GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H319 |
| Precautionary Statements | P305 + P351 + P338 |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
| Hazard Codes | Xi:Irritant; |
| Risk Phrases | R36;R43 |
| Safety Phrases | S22-S24/25-S37/39-S26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| RTECS | LM5075000 |
| Packaging Group | I; II; III |
| HS Code | 3204200000 |
Customs
| HS Code | 2932999099 |
|---|---|
| Summary | 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
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Synonyms
| D and C Yellow No. 7 |
| D&C Yellow No. 7 |
| D+C Yellow No. 7 |
| Fluorescein (free acid) |
| 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one |
| 3',6'-Fluorandiol |
| Fluorecein |
| Eosin |
| YELLOW 7 |
| 3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one |
| KI201 |
| MFCD00005050 |
| Acid Yellow 73 |
| 3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one |
| Fluorescite |
| EINECS 219-031-8 |
| Uranine Yellow |
| C.I. Acid Yellow 73 |
| angiofluor |
| Soluble fluorescein |
| D & C Yellow no. 7 |
| Fluor-I-Strip |
| D & C Yellow no. 8 |
| Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy- |
| Fluorescein |
| Ful-Glo |
| Funduscein |
