3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester CAS 35286-58-9

Introduction：Basic information about 3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester CAS 35286-58-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester Basic information

• Product Name: 3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester
• Synonyms: Ulmus pumila saponin I;3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester;Kudinoside H;Zigu-glucoside I;Gouguside 7;Ziyu-glycoside Ⅰ;Urs-12-en-28-oic acid, 3-(α-L-arabinopyranosyloxy)-19-hydroxy-, β-D-glucopyranosyl ester, (3β)-;[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (1R,2R,4aS,6aR,6aS,6bR,8aR,10S,12aR,14bS)-1-hydroxy-1,2,6a,6b,9,9,12a-heptamethyl-10-[(2S,3R,4S,5S)-3,4,5-trihydroxyoxan-2-yl]oxy-2,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylate
• CAS: 35286-58-9
• MF: C41H66O13
• MW: 766.95
• EINECS: 233-305-4
• Product Categories: chemical reagent;pharmaceutical intermediate;phytochemical;reference standards from Chinese medicinal herbs (TCM).;standardized herbal extract
• Mol File: 35286-58-9.mol

3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester Chemical Properties

• Melting point: 198-202 ºC
• Boiling point: 841.9±65.0 °C(Predicted)
• density: 1.35
• storage temp.: -20°C, protect from light
• solubility: Soluble in acetonitrile and methanol;
• form: powder
• pka: 12.52±0.70(Predicted)
• color: White
• Water Solubility: insoluble in water
• InChIKey: WCHBFWOEFOZHMK-MLHVESHNSA-N

Safety Information

• WGK Germany: WGK 3
• Storage Class: 11 - Combustible Solids

3-O-alpha-L-Arabinopyranosylpomolic acid beta-D-glucopyranosyl ester Usage And Synthesis

Chemical Properties

White crystals, soluble in methanol, ethanol, DMSO and other organic solvents, derived from Burnet.

Uses

Used for content determination/identification/pharmacological experiments, etc.

Biological Activity

Ziyuglycoside I, isolated from the roots of S. officinalis, has anti-wrinkle activity and increases collagen type I expression. It can be used as an active ingredient in cosmetics and induces p53-mediated cell cycle arrest and apoptosis (apoptosis), which can be used in the study of triple-negative breast cancer.

Physiological effects

Ziyuglycoside I has the functions of vasoconstriction, hemostasis and antifungal.

in vitro

Ziyuglycoside I (5-160 μM; 24 hours) reveals a marked anti-proliferation activity with an IC ₅₀ value of 13.96 μM in MDA-MB-231 cells. Ziyuglycoside I ( 5-20 μM; 24 hours) induces MDA-MB-231 cell apoptosis by increasing the percentage of apoptotic cells from 2.43% to 44.76% at 20 μM. Ziyuglycoside I (5-20 μM; 24 hours) induces the cleavage of caspas- 8, caspase-9, and caspase-3, up-regulates the pro-apoptotic protein Bax, and down-regulates anti-apoptotic protein Bal-2, dose-dependently reduces the level of mito-cyto c expression.

Cell Viability Assay

Cell Cycle Analysis

Western Blot Analysis

• Cell Line: MDA-MB-231 cells < /td>
• Concentration: 5 μM, 10 μM, 20 μM, 40 μM, 80 μM, and 160 μM
• Incubation Time: 24 hours
• Result: Showed cytotoxicity on MDA-MB-231 cells.

• Cell Line: MDA-MB-231 cells
• Concentration: 5 μM, 10 μM, and 20 μM
• Incubation Time: 24 hours
• Result: Induced G2/M phase arrest and apoptosis on MDA-MB-231 Cells

• Cell Line: MDA-MB-231 cells
• Concentration: 5 μM, 10 μM, and 20 μM
• Incubation Time: 24 hours
• Result: Induced apoptosis in MDA-MB-231 Cells through Intrinsic and Extrinsic Pathways.