Introduction：Basic information about 4-Chloro-7-nitrobenzofurazan CAS 10199-89-0, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
4-Chloro-7-nitrobenzofurazan Basic information
| Product Name: | 4-Chloro-7-nitrobenzofurazan |
| Synonyms: | 4-CHLORO-7-NITROBENZOFURAZAN, FOR FLUORE SCENCE;7-CHLORO-4-NITROBENZ-2-OXA-1-3-DIAZOLECR YSTALLINE;4-Chloro-7-nitrobenzo-2,1,3-oxadiazole~NBDchloride;2,1,3-Benzoxadiazole, 4-chloro-7-nitro-;4-chloro-7-nitrobenzo-2,1,3-oxadiazole;NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling];4-chloro-7-nitrobenzofurazane;4-Chloro-7-nitro-1,2,3-benzoxadiazole, NBD-chloride |
| CAS: | 10199-89-0 |
| MF: | C6H2ClN3O3 |
| MW: | 199.55 |
| EINECS: | 233-496-4 |
| Product Categories: | Functional Group Reactive Labels for Immunoassays;Biochemicals and Reagents;Miscellaneous;Amino Group Labeling Reagents for Fluorescence HPLC;Analytical Chemistry;Fluorescence Detection (HPLC Labeling Reagents);HPLC Labeling Reagents;Mercapto Group Labeling Reagents for Fluorescence HPLC;Pharmaceutical intermediate;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Building Blocks;Chemical Synthesis;Derivatization agents;Fluorescent Probes;Functional Group Reactive Labels;Halogenated Heterocycles;Heterocyclic Building Blocks;Labels;Luminescent Compounds/Detection;Oxadiazoles;Particles and Stains |
| Mol File: | 10199-89-0.mol |
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4-Chloro-7-nitrobenzofurazan Chemical Properties
| Melting point | 97-99 °C(lit.) |
| Boiling point | 333.1±45.0 °C(Predicted) |
| density | 2.0589 (rough estimate) |
| refractive index | 1.5810 (estimate) |
| storage temp. | Inert atmosphere,Room Temperature |
| solubility | Soluble in methanol, dimethylsulfoxide, dimethylformamide and chloroform. |
| form | Crystalline Powder |
| pka | -5.65±0.50(Predicted) |
| color | Yellow to light brown |
| Water Solubility | soluble |
| BRN | 614212 |
| InChI | InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H |
| InChIKey | IGHBXJSNZCFXNK-UHFFFAOYSA-N |
| SMILES | N1=C2C([N+]([O-])=O)=CC=C(Cl)C2=NO1 |
| CAS DataBase Reference | 10199-89-0(CAS DataBase Reference) |
| EPA Substance Registry System | 2,1,3-Benzoxadiazole, 4-chloro-7-nitro- (10199-89-0) |
Safety Information
| Hazard Codes | Xn,Xi |
| Risk Statements | 22-36/38 |
| Safety Statements | 36/37/39 |
| RIDADR | UN 2811 6.1/PG 3 |
| WGK Germany | 3 |
| RTECS | DF8002400 |
| F | 10-21 |
| TSCA | TSCA listed |
| HS Code | 29349990 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Eye Irrit. 2
Skin Irrit. 2 |
4-Chloro-7-nitrobenzofurazan Usage And Synthesis
| Chemical Properties | yellow to light brown crystalline powder |
| Uses | 4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N-terminals within proteins and trapping agent for cysteine sulfenic acid. It is a sensitive chromogenic and fluorogenic reagent. It reacts with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky's diene) to prepare regioselectively the silyl enol ether. Further, it is used to label peptides, proteins and other biomolecules. In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide. |
| Uses | 4-Chloro-7-nitrobenzofurazan is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. 4-Chloro-7-nitrobenzofurazan has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. |
| Definition | ChEBI: A benzoxadiazole that is 2,1,3-benzoxadiazole which is substituted at position 4 by chlorine and at position 7 by a nitro group. |
| General Description | 4-Chloro-7-nitrobenzofurazan (NBD-Cl) is a highly sensitive chromogenic and fluorogenic reagent. It is reported to react spontaneously with trans-1-methoxy-3-(trimethylsilyloxy)-1,3-butadiene (Danishefsky′s diene), to afford regioselectively the silyl enol ether, via normal electron-demand Diels-Alder (NEDDA) reaction. A study of the molecular structure, vibrational spectra and NBO (Natural Bond Orbital) analysis of NBD–Cl has been undertaken. Its utility as a pre-column derivatization agent has been examined. |
| in vitro | 4-Chloro-7-nitrobenzofurazan(NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides. |
| Purification Methods | Wash the solid with H2O, and it recrystallises from aqueous EtOH (1:1) as pale yellow needles. It sublimes in a vacuum [Gosh & Whitehouse Biochem J 108 155 1968, UV, NMR: Bolton et al. J Chem Soc 1004 1966]. |
4-Chloro-7-nitrobenzofurazan Preparation Products And Raw materials
| Preparation Products | 4-Nitro-7-piperidin-1-yl-2,1,3-benzoxadiazole-->2-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)thio]ethanaMine |
