5-Aminofluorescein CAS 3326-34-9
Introduction：Basic information about 5-Aminofluorescein CAS 3326-34-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
5-Aminofluorescein Basic information
| Product Name: | 5-Aminofluorescein |
| Synonyms: | 5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-oneFluoresceinamine;5-amino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one;FLUORESCEINAMINE;FLUORESCEINAMINE ISOMER I;FLUORESCEINAMINE, ISOMER 1;5-amino-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid;5-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE;5-AMINOFLUORESCEIN ISOMER I |
| CAS: | 3326-34-9 |
| MF: | C20H13NO5 |
| MW: | 347.32 |
| EINECS: | 222-043-6 |
| Product Categories: | marker;Fluorescent Labels and Indicators;Fluorescent Labels & Indicators |
| Mol File: | 3326-34-9.mol |
5-Aminofluorescein Chemical Properties
| Melting point | 223 °C (dec.)(lit.) |
| Boiling point | 481.89°C (rough estimate) |
| density | 1.3724 (rough estimate) |
| refractive index | 1.5180 (estimate) |
| storage temp. | room temp |
| solubility | DMF: 5 mg/ml; DMSO: 5 mg/ml |
| pka | 9.34±0.20(Predicted) |
| form | powder |
| color | red-brown |
| Appearance | Solid Powder |
| Water Solubility | Soluble in water (partly), methanol (10 mg/ml), and acetone. |
| λmax | 496 nm |
| BRN | 48395 |
| Stability: | Light and Moisture Sensitive |
| Major Application | diagnostic assay manufacturing hematology histology |
| InChI | 1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2 |
| InChIKey | GZAJOEGTZDUSKS-UHFFFAOYSA-N |
| SMILES | Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35 |
| CAS DataBase Reference | 3326-34-9(CAS DataBase Reference) |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5-amino-3',6'-dihydroxy- (3326-34-9) |
Safety Information
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26 |
| WGK Germany | 3 |
| F | 10 |
| TSCA | TSCA listed |
| HS Code | 32049090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Acute Tox. 4 Oral Eye Irrit. 2 Skin Irrit. 2 STOT SE 3 |
| Description | 5-AMF is a fluorescent probe for carboxylic acids and glutamine, and it also can be coupled to aldehydes and ketones. |
| Chemical Properties | Dark Red Solid |
| Uses | Glycosaminoglycuronans react with 5-aminofluorescein to yield fluorescent derivatives. 5-aminofluorescein is also used to prepare FITC Isomer I (F-011, F-020). |
| Uses | 5-aminofluorescein is also used to prepare FITC Isomer I (CDX-F0011, CDX-F0020). It is a chemical used as molecular probe and important as fluorescent dye and for derivatives. Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. |
| Definition | ChEBI: 5-aminofluorescein is a primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid. It derives from a fluorescein (lactone form). |
| Preparation | The preparation of 5-aminofluorescein is carried out either by first saponification of 5-nitrofluorescein acetates with alcohol alkali, followed by reduction of the obtained 5-nitrofluorescein in a solution of alcohol alkali with hydrazine hydrate on a nickel catalyst to obtain 5-aminofluorescein , Or by boiling in an aqueous solution of sodium sulfide. The restoration of the nitro group and the saponification of acetates in the second case occurs in one stage. |
| General Description | Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins. |
5-Aminofluorescein Preparation Products And Raw materials
| Raw materials | Sodium sulfide-->Resorcinol-->Sodium hydrosulfide-->4-Nitrophthalic acid-->4-AMINO-N-METHYLPHTHALIMIDE-->6-Aminofluorescein |
| Preparation Products | Fluorescein isothiocyanate-->azidofluorescein |
