Introduction：Basic information about 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside CAS 7240-90-6, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Basic information
| Product Name: | 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside |
| Synonyms: | X-BETA-D-GAL;X-BETA-D-GALACTOSIDE;X-BETA-GAL;X-GAL READY TO USE;X-GAL;5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOPYRANOSIDE;5-BROMO-4-CHLORO-1H-INDOL-3-YL BETA-D-GALACTOSIDE-(1,5);5-BROMO-4-CHLORO-3-INDOLYL-B B-D-GALACTOPYRANOSIDE |
| CAS: | 7240-90-6 |
| MF: | C14H15BrClNO6 |
| MW: | 408.63 |
| EINECS: | 230-640-8 |
| Product Categories: | Fluorescent Labels & Indicators;Labeling and Diagnostics Reagents;Enzyme substrates;Carbohydrates & Derivatives;substrate |
| Mol File: | 7240-90-6.mol |
|
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Chemical Properties
| Melting point | 230 °C |
| alpha | -64 º (C=1, H2O/DMF (1/1)) |
| Boiling point | 673.9±55.0 °C(Predicted) |
| density | 1.5563 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | -20°C |
| solubility | Soluble in 100 mM in DMSO. |
| form | Powder |
| pka | 12.74±0.70(Predicted) |
| color | White |
| Odor | Odorless |
| Sensitive | Moisture & Light Sensitive |
| Merck | 14,10074 |
| BRN | 1402009 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| InChI | 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1 |
| InChIKey | OPIFSICVWOWJMJ-AEOCFKNESA-N |
| SMILES | OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O |
| CAS DataBase Reference | 7240-90-6(CAS DataBase Reference) |
Safety Information
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 24/25-37/39-26 |
| WGK Germany | 3 |
| F | 8-10-21 |
| HazardClass | IRRITANT |
| HS Code | 29389090 |
| Storage Class | 11 - Combustible Solids |
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Usage And Synthesis
| Chemical Properties | White Powder |
| Uses | A substrate for β-Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm). |
| Uses | magenta b-galactosidase substrate |
| Uses | In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments. |
| Uses | A substrate for -Galactosidase that is converted by the enzyme into an intense indigo-blue chromophore (615 nm) |
| Definition | ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product. |
| Biological Activity | X-gal is routinely used to detect transgene activity in situ. |
| storage | +4°C |
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside Preparation Products And Raw materials
