Acid Blue 83 CAS 6104-59-2

Introduction：Basic information about Acid Blue 83 CAS 6104-59-2, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Acid Blue 83 Basic information

• Product Name: Acid Blue 83
• Synonyms: SERVA BLUE R;SERVA BLUE R STAINING SOLUTION;SOLAR CYANINE 6B;R STANDS FOR ''REDDISH'';PAGE BLUE 83;Coomassie brilliant R250;Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl) amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino ]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]- N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium salt;Brilliant Blue 6B
• CAS: 6104-59-2
• MF: C45H46N3NaO7S2
• MW: 827.99
• EINECS: 228-060-5
• Product Categories: Dyes and Pigments;Organics;Biochemistry;Reagents for Electrophoresis
• Mol File: 6104-59-2.mol

Acid Blue 83 Chemical Properties

• Melting point: 174-180 °C
• bulk density: 250kg/m3
• vapor density: 28.5 (vs air)
• Fp: 14 °C
• storage temp.: room temp
• solubility: H2O: soluble10mg/mL
• form: Powder/Solid
• Colour Index: 42660
• color: Red Brown
• PH: 6.2 (25°C, 10g/L in H2O)
• Water Solubility: Soluble in water.
• λmax: 589.2 nm in ethanol
• BRN: 5718025
• Stability: Light Sensitive
• Major Application: diagnostic assay manufacturing hematology histology
• InChIKey: NKLPQNGYXWVELD-UHFFFAOYSA-M
• SMILES: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• CAS DataBase Reference: 6104-59-2(CAS DataBase Reference)
• EPA Substance Registry System: C.I. Acid Blue 83 (6104-59-2)

Safety Information

• WGK Germany: 3
• TSCA: TSCA listed
• HS Code: 32041200
• Storage Class: 11 - Combustible Solids

Acid Blue 83 Usage And Synthesis

Chemical Properties	dark bordeaux to dark purplish-brown cryst. powder
Uses	Brilliant Blue R is used for detection of protein bands following electrophoresis. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. It displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.
Uses	Acid Blue 83 is a dye commonly used in laboratories or quantify proteins.
Preparation	p-Chlorobenzaldehyde?(1 Moore) and 3-(Ethyl(phenyl)amino)methyl)benzenesulfonic acid (2 Moore) condensation, and heavy chromate salt-oxalic acid oxidation its product, again with 4-Ethoxybenzenamine condensation.
Biochem/physiol Actions	Brilliant Blue R is a methanol based stain. It can also stain agarose gels. It consists of methanol and acetic acid.
storage	Room temperature
Properties and Applications	blue. Dark blue powder. Not soluble in cold water, slightly soluble in hot water and ethanol for light blue. The strong sulfuric acid for orange red, diluted into blue. In water solution to join sodium hydroxide purple precipitation. Used for wool, silk, polyamide fiber fabric dyeing and printing directly, can also be used for leather and feathers dyeing. Standard Light Fastness Soaping Persperation Fastness Oxygen bleaching Fastness to seawater Fading Stain Fading Stain Fading Stain ISO 3 4 4 4 1 1-2 4 4-5 AATCC 2-3 3-4 3-4 4-5 1 1-2 4-5 4-5

Acid Blue 83 Preparation Products And Raw materials

• Raw materials: 4-Chlorobenzaldehyde-->Potassium dichromate-->Benzenesulfonic acid-->Phenetidine-->4-((Ethylanilino)methyl)benzenesulphonic acid-->Phenetidine-->4-Chlorobenzaldehyde-->N-Ethyl-N-benzylaniline-3'-sulfonic acid