Introduction：Basic information about Alkaline Phosphatase CAS 9001-78-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Alkaline Phosphatase Basic informationDescription Source
| Product Name: | Alkaline Phosphatase |
| Synonyms: | AlkalinePhosphatase,(AmmoniumSulfateSuspensionCalfIntestine);ALKALINE PHOSPHATASE, RECOMBINANT, MOLECULAR BIOLOGY GRADE;ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT;Alkaline Phosphatase from calf intestine ca. 3 000 U/mg protein (ca. 60 U/;Alkaline Phosphatase from calf intestine ca. 5 400 U/mg protein;Alkaline Phosphatase from calf intestine ca. 7000 U/mg protein;alkaline phosphatase from calf intestinal mucosa;phosphatase, alkaline from escherichia coli |
| CAS: | 9001-78-9 |
| MF: | C21H36N8O6 |
| MW: | 496.56054 |
| EINECS: | 232-631-4 |
| Product Categories: | HPLC Fittings;SSI Fittings;Stainless Steel Fittings;enzyme;CAP |
| Mol File: | 9001-78-9.mol |
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Alkaline Phosphatase Chemical Properties
| density | 1.12 g/mL at 20 °C |
| storage temp. | -20°C |
| form | suspension |
| color | white |
| Specific Gravity | 1 |
| PH | ~7.6 |
| biological source | mouse |
| Water Solubility | It is soluble in water. |
| Specific Activity | 30-90units/mg protein (modified Warburg-Christian, in glycine buffer) |
| Stability: | Stability Incompatible with strong oxidizing agents |
| Cosmetics Ingredients Functions | SKIN CONDITIONING |
| InChIKey | ITZMJCSORYKOSI-XUAWLZQMNA-N |
| SMILES | C(O)(=O)[C@@H](NC([C@@H]1CCCN1C(CNC([C@@H]1CCCN1C(=O)[C@@H](N)C)=O)=O)=O)CCC/N=C(\N)/N |&1:3,6,15,22,r| |
| EPA Substance Registry System | Alkaline phosphatase (9001-78-9) |
| Absorption | 7.6 at 278nm (10 mg AP/ml) |
Safety Information
| Hazard Codes | B,Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36-24/25-22-23 |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | TSCA listed |
| HS Code | 35079090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Resp. Sens. 1 |
| Toxicity | An enzyme with analkaline pH optimum that hydrolyzes phosphate monoesters.Elevation of its activity in the serum usually indicates obstructivejaundice, Paget’s disease (osteitis deformans), or bonecarcinoma. |
Alkaline Phosphatase Usage And Synthesis
| Chemical Properties | solid |
| Uses | Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes. |
| Uses | Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids. |
| Uses | Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions. |
| General Description | Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. |
| Biochem/physiol Actions | Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA). |
| Purification Methods | The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.] |
| Description | The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. |
| Source | Calf Intestine |
Alkaline Phosphatase Preparation Products And Raw materials
