Introduction：Basic information about GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE CAS 125455-61-0, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE Basic information
| Product Name: | GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE |
| Synonyms: | H-GLY-ARG-PNA 2HCL;GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE;H-Gly-Arg-Pna dihydrochloride;Gly-Arg-4-NA;GLY-ARG-4-NA;GR-PNAGR; P-NITROANILIDE;GR-pNAGR;Gly-Arg-p-nitroanilide dihydrochloride unokinase and plasmin substrate |
| CAS: | 125455-61-0 |
| MF: | C14H23Cl2N7O4 |
| MW: | 424.28 |
| EINECS: | |
| Product Categories: | Coagulation Proteins and ReagentsEnzyme Substrates;Plasma&Blood Proteins;PlasminEnzyme Substrates;Protease Substrates;Proteins and Derivatives;Substrates by Enzyme;Urokinase;UrokinaseEnzyme Substrates |
| Mol File: | 125455-61-0.mol |
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GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE Chemical Properties
| storage temp. | −20°C |
| solubility | DMF: 20mg/mL; DMSO: 3mg/mL; PBS (pH 7.2): 3mg/mL |
| form | A solid |
| InChIKey | UMNMBKCWESYUMR-LCJJWKFENA-N |
| SMILES | C1(C=CC([N+]([O-])=O)=CC=1)NC(=O)[C@@H](NC(=O)CN)CCCNC(=N)N.Cl.Cl |&1:12,r| |
Safety Information
GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE Usage And Synthesis
| Description | H-Gly-Arg-pNA is a colorimetric substrate for thrombin. Thrombin preferentially binds to and cleaves the Gly-Arg (GR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity. |
| Uses | H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity[1]. |
| References | [1] Hortin G L, et al. Macromolecular chromogenic substrates for measuring proteinase activity[J]. Clinical chemistry, 2001, 47(2): 215-222. PMID:11159769 |
GLY-ARG P-NITROANILIDE DIHYDROCHLORIDE Preparation Products And Raw materials
