Introduction：Basic information about Neutral Red CAS 553-24-2, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Neutral Red Basic information
| Product Name: | Neutral Red |
| Synonyms: | AMinodiMethylaMinotoluaMinozine;Neutral Red, high purity biological stain, pure 25GR;NEUTRAL RED (C.I. 50040) INDICATOR AND F;NEUTRAL RED (C.I. 50040) FOR MICROSCOPY;Neutral Red, Reagent, ACS;Neutral Red, high purity biological stain;Neutral Red, indicator (pH 6.8 - 8.0);N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride |
| CAS: | 553-24-2 |
| MF: | C15H17ClN4 |
| MW: | 288.78 |
| EINECS: | 209-035-8 |
| Product Categories: | Phenazine;Azine;Organics;Analytical Chemistry;marker;Miscellaneous;Indicator (pH);pH Indicators;Stains and Dyes |
| Mol File: | 553-24-2.mol |
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Neutral Red Chemical Properties
| Melting point | 290 °C (dec.) (lit.) |
| Boiling point | 441.31°C (rough estimate) |
| bulk density | 350-500kg/m3 |
| density | 1.1590 (rough estimate) |
| refractive index | 1.6110 (estimate) |
| storage temp. | Store at +5°C to +30°C. |
| solubility | H2O: soluble10mg/mL |
| pka | 6.7, 7.4(at 25℃) |
| form | Solid |
| Colour Index | 50040 |
| color | Very dark green |
| Appearance | Solid Powder |
| Odor | Odorless |
| PH | 3.1 (10g/l, H2O, 20℃) |
| PH Range | 6.8(red)-8(yellow) |
| Water Solubility | 50 g/L |
| λmax | 540nm, 533nm, 544nm, 529nm, 454nm |
| Merck | 14,6488 |
| BRN | 3918740 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| Major Application | Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases |
| Biological Applications | Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases |
| InChI | 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H |
| InChIKey | PGSADBUBUOPOJS-UHFFFAOYSA-N |
| SMILES | Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1 |
| CAS DataBase Reference | 553-24-2(CAS DataBase Reference) |
| EPA Substance Registry System | Neutral Red (553-24-2) |
Safety Information
| Hazard Codes | Xn,Xi |
| Risk Statements | 68-36/37/38 |
| Safety Statements | 36/37-37/39-36-26-24/25 |
| WGK Germany | 3 |
| RTECS | SG1400000 |
| TSCA | TSCA listed |
| HS Code | 32041300 |
| Storage Class | 11 - Combustible Solids |
| Toxicity | LD50 intraperitoneal in mouse: 432mg/kg |
Neutral Red Usage And Synthesis
| Description | Neutral red is useful as a biological stain and as an indicator for preparing neutral red paper. |
| Chemical Properties | dark green powder |
| Uses | Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites. |
| Uses | Can be used as a vital stain, to stain living cells. |
| Application | Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it. |
| Definition | ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+). |
| Preparation | (a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture. |
| General Description | Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl. |
| Biochem/physiol Actions | Neutral red is a widely used cationic fluorochrome that is known to accumulate in the Golgi complex and nuclei after first staining lysosomes. |
| Biotechnological Applications | Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls. |
| storage | +4°C |
| Staining Procedures | Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls. |
| Properties and Applications | red light purple and red. Soluble in water is cherry red, soluble in ethanol for product red colour with red fluorescent light brown. The strong sulfuric acid for green, blue for diluted, and then into a product red. The dye solution to join strong sodium hydroxide solution have yellow brown precipitation. |
| Purification Methods | In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.] |
Neutral Red Preparation Products And Raw materials
| Raw materials | 2,4-Diaminotoluene-->N,N-DIMETHYL-4-NITROSOANILINE-->N,N-Dimethyl-1,4-phenylenediamine |
