Peroxidase CAS 9003-99-0

Introduction：Basic information about Peroxidase CAS 9003-99-0, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Peroxidase Basic information

• Product Name: Peroxidase
• Synonyms: Peroxidase froM horseradish(EIA Grade,Purified);84 kDa myeloperoxidase;89 kDa myeloperoxidase;ANTI-MYELOPEROXIDASE(N-TERMINAL) antibody produced in rabbit;Myeloperoxidase light chain;Apoperoxidase;Peroxidase isoenzymes Type XI: Apoenzyme from horseradish;ANTI-MYELOPEROXIDASE (CENTER) antibody produced in rabbit
• CAS: 9003-99-0
• MF: NULL
• MW: 0
• EINECS: 232-668-6
• Product Categories: Alkaline Phosphatase ConjugatesWestern Blotting;Antibody Conjugates;Peroxidase ConjugatesAntibodies;Protein A, G and L Detection Reagents;Secondary Antibodies;Western Blotting;Biochemistry;Enzyme;Oxido-Reductase;Enzymes;Oxidoreductases;Specialty Enzymes
• Mol File: Mol File

Peroxidase Chemical Properties

• density: 1.37[at 20℃]
• vapor pressure: 0.004Pa at 25℃
• storage temp.: -20°C
• solubility: H₂O: soluble
• form: powder
• color: red-brown
• PH: 6.5-7.5 (20°C, 10g/L in H2O)
• biological source: human leucocytes
• Water Solubility: Soluble in water and phosphate buffer.
• Specific Activity: 200-300units/mg solid (using pyrogallol)
• Cosmetics Ingredients Functions: SKIN CONDITIONING ANTIOXIDANT LIGHT STABILIZER
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChIKey: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• SMILES: [O+H2]O[O-]
• LogP: -1.3 at 20℃
• EPA Substance Registry System: Peroxidase (9003-99-0)

Safety Information

• Hazard Codes: B
• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• TSCA: TSCA listed
• HS Code: 35079090
• Storage Class: 11 - Combustible Solids
• Hazard Classifications: Resp. Sens. 1 Skin Sens. 1

Peroxidase Usage And Synthesis

Chemical Properties

Brown crystalline substance or lyophilized powder, soluble in water, commercial ammonium sulfate suspension, pI7.2, optimum pH 7.0.Stability: lyophilized powder and 2.8 mol/L ammonium sulfate suspension at 4℃ can be stable for one year. The solution has maximum absorption at 275nm, 403nm and 500nm. Enzyme reaction: donor + H2O ═ oxidized donor ten 2H2O.

Uses

lactoperoxidase is a glycoprotein with anti-microbial activity, it is used as a stabilizing ingredient to help improve formulation stability and product shelf-life. It is naturally occurring in milk.

Uses

Peroxidases (EC 1.11.1.x) are a diverse group of oxidoreductases using peroxideas an electron acceptor. Their substrate spectrum ranges from hydrogen peroxidedecomposed by catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase,EC 1.11.1.6), phenolic compounds degraded by Mn-dependent and lignin peroxidases(Mn(II):hydrogen-peroxide oxidoreductase, EC 1.11.1.13 and 1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol:hydrogen-peroxide oxidoreductase, EC1.11.1.14), and recalcitrant dyes by DyP-type peroxidases (reactive-blue-5:hydrogen-peroxide oxidoreductase, EC 1.11.1.19).
Direct supplementation of peroxidases to food was applied for the degradationof carotenoids used for coloring of cheese. The whey resulting from coloredcheese production has an orange-yellowish tint, which interferes with further usageof the whey. Recently, a fungal peroxidase of the DyP-type was commercializedfor bleaching of this kind of whey fluid under the name MaxiBright (DSM). Togenerate the hydrogen peroxide required by the peroxidase as a cofactor in situ, aglucose oxidase and a b-galactosidase were employed as auxiliary enzymes.

Uses

Peroxidase, horseradish is used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody. Further, it is highly reactive toward human tumor cells.

Definition

ChEBI: N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide is a member of benzamides.

Biological Functions

Peroxidases are widely distributed in plants, animals, and microorganisms, and they protect cells against the effects of oxidative stress and cell damage due to H2O2. Peroxidases (EC number 1.11.1.x) represent a large family of oxidoreductases known to catalyze the oxidation of various inorganic and organic substrates using H2O2. They have a common catalytic mechanism for the degradation of H2O2, a two-electron oxidation-reduction with three distinct steps. They are involved in biological processes such as the breakdown of toxins, heavy metal detoxification and hormone regulation. Additionally, they are used as catalysts in various industries, in clinical biochemistry, enzyme immunoassays, the removal of peroxides from industrial wastes and the synthesis of various aromatic chemicals[1].

General Description

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains. Peroxidase from Streptococcus sp. is used to maintain the H₂O₂ homeostasis in cells. It aids the regulation of ABC Mn²⁺-permease complex (psaBCA) genes.

Flammability and Explosibility

Not classified

Biochem/physiol Actions

The transformation of industrial dyes by manganese peroxidases from Bjerkandera adusta is a manganese-independent reaction .

Synthesis

(1) Horseradish was crushed, macerated, and centrifuged to obtain a crude extract of horseradish peroxidase;

(2) the crude extract of horseradish peroxidase was separated by ultrafiltration using ultrafiltration membrane with a molecular weight cut-off of 70kDa-100kDa to obtain the ultrafiltrate of horseradish peroxidase;

(3) the horseradish peroxidase ultrafiltration solution was concentrated by ultrafiltration using an ultrafiltration membrane with a molecular weight cut-off of 1-30 kDa to obtain horseradish peroxidase concentrate;

(4) Dehydrate the horseradish peroxidase concentrate to obtain horseradish peroxidase.

References

[1] Pontsho Patricia Twala. “Breakthroughs in the discovery and use of different peroxidase isoforms of microbial origin.” AIMS Microbiology 6 3 (2020): 330–349.

Peroxidase Preparation Products And Raw materials

• Raw materials: Ammonium sulfate-->Calcium phosphate-->Horseradish, ext.