Introduction：Basic information about CAS 138777-25-0|C12FDG, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | C12FDG |
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| CAS Number | 138777-25-0 | Molecular Weight | 853.90500 |
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| Density | / | Boiling Point | / |
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| Molecular Formula | C44H55NO16 | Melting Point | / |
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| MSDS | / | Flash Point | / |
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Names
| Name | N-[3-oxo-3',6'-bis[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-1,9'-xanthene]-5-yl]dodecanamide |
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| Synonym | More Synonyms |
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C12FDG BiologicalActivity
| Description | C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells[1]. |
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| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
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| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Fluorescent senescence-associated β-galactosidase (SA-β-Gal) assay[2]: 1. Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL overnight. Incubate the cells according to your normal protocol. 2. Wash cells by 200 μL of PBS once and fix with 100 μL of fixation solution (2% formaldehyde/0.2% glutaraldehyde in distilled water) at room temperature for 5 min. 3. Wash cells by 200 μL of PBS two times, and stain with 100 μL of 33 μM C12FDG (in PBS, pH=6.0) for 10 min, and with 200 μL of Hoechst solution (1 μg/mL Hoechst 33342 (HY-15559) in PBS, pH 6.0) for 10 min. 4. Image these cells by a 20× objective and 360-nm (Hoechst 33342) and 480-nm (C12FDG) excitation filters, and monitor through 460-nm and 535-nm emission filters, respectively. |
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| References | [1]. Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41. [2]. Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8. |
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Chemical & Physical Properties
| Molecular Formula | C44H55NO16 |
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| Molecular Weight | 853.90500 |
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| Exact Mass | 853.35200 |
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| PSA | 266.88000 |
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| LogP | 3.12090 |
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| InChIKey | SLIWIQKBDGZFQR-PIVCGYGYSA-N |
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| SMILES | CCCCCCCCCCCC(=O)Nc1ccc2c(c1)C(=O)OC21c2ccc(OC3OC(CO)C(O)C(O)C3O)cc2Oc2cc(OC3OC(CO)C(O)C(O)C3O)ccc21 |
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| Storage condition | -20°C |
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Synonyms
| 5-Dodecanoylaminoflorescein di-b-D-galactopyranoside |
| W0468 |
