CAS 312756-74-4|RS-1
| Common Name | RS-1 | ||
|---|---|---|---|
| CAS Number | 312756-74-4 | Molecular Weight | 524.22600 |
| Density | / | Boiling Point | / |
| Molecular Formula | C20H16Br2N2O3S | Melting Point | / |
| MSDS | USA | Flash Point | / |
Names
| Name | 3-(Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide |
|---|---|
| Synonym | More Synonyms |
RS-1 BiologicalActivity
| Description | RS-1 is a RAD51 activator, and also increases CRISPR/Cas9-mediated knock-in efficiencies. |
|---|---|
| Related Catalog | Signaling Pathways >>Cell Cycle/DNA Damage >>CRISPR/Cas9Signaling Pathways >>Cell Cycle/DNA Damage >>RAD51Research Areas >>Cancer |
| Target | RAD51[1], CRISPR/Cas9[2] |
| In Vitro | RS-1 is a RAD51 activator, stimulating binding of hRAD51 to DNA with Kd ranging from 48 nM to 107 nM in the presence of ATP or ADP and in the absence of a nucleotide cofactor, and such an effect is not via inhibiting its ATPase activity. RS-1 (20 μM) affects the length and helical pitch of hRAD51 protein-DNA complexes. RS-1 (0, 1, 5, 10, 15, 20, and 25 μM) stimulates strand assimilation activity of hRAD51. RS-1 (7.5 μM) promotes resistance of human cells to cross-linking chemotherapy[1]. RS-1 (0, 7.5, 15 μM) increases Cas9-mediated knock-in efficiencies in rabbit embryos[2]. |
| Kinase Assay | Briefly, 15 μL reaction volumes include a DNA strand exchange protein (0.8 μM) that is preincubated for 5 min at 37°C with 1 μM (nucleotide concentration) 32P-labeled oligonucleotide 306.7 in a reaction buffer containing 20 mM Hepes (pH 7.5), 1 mM DTT, 2 mM nucleotide cofactor, and 1 mM MgCl2 and various concentrations of RS-1. For experimental buffer conditions that included calcium, 1 mM CaCl2 is present in addition to (in the case of hRAD51) or in the place of (in the case of RecA and scRAD51) the 1 mM MgCl2. Conditions with scRAD51 additionally contains 110 nM scRAD54. After this initial binding reaction, 10 μL of 19.75 μM (base pair concentration) supercoiled homologuecontaining target plasmid DNA (pRS306) is next added along with sufficient magnesium acetate to give a final concentration of 10 mM[1]. |
| References | [1]. Jayathilaka K, et al. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53. [2]. Song J, et al. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548. |
Chemical & Physical Properties
| Molecular Formula | C20H16Br2N2O3S |
|---|---|
| Molecular Weight | 524.22600 |
| Exact Mass | 521.92500 |
| PSA | 83.65000 |
| LogP | 6.48710 |
| InChIKey | SWKAVEUTKGKHSR-UHFFFAOYSA-N |
| SMILES | O=C(Nc1ccc(Br)cc1)c1ccc(Br)c(S(=O)(=O)NCc2ccccc2)c1 |
| Storage condition | -20℃ |
Safety Information
| RIDADR | NONH for all modes of transport |
|---|
Articles3
More Articles| RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat. Commun. 7 , 10548, (2016) Zinc-finger nuclease, transcription activator-like effector nuclease and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) are becoming major tools ... | |
| Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Nucleic Acids Res. 43(19) , 9379-92, (2015) CRISPR is a genome-editing platform that makes use of the bacterially-derived endonuclease Cas9 to introduce DNA double-strand breaks at precise locations in the genome using complementary guide RNAs.... | |
| A chemical compound that stimulates the human homologous recombination protein RAD51. Proc. Natl. Acad. Sci. U. S. A. 105 , 15848-15853, (2008) RAD51 and other members of the RecA family of strand exchange proteins assemble on ssDNA to form presynaptic filaments, which carry out the central steps of homologous recombination. A microplate-base... |
Synonyms
| 3-[(benzylamino)sulfonyl]-4-bromo-N-(4-bromophenyl)benzamide |
| RAD51-stimulatory compound 1 |
| RS-1 |
| 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide |
