Introduction：Basic information about CAS 130926-94-2|Mag-indo-1/AM, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Mag-indo-1/AM |
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| CAS Number | 130926-94-2 | Molecular Weight | 612.58100 |
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| Density | 1.335g/cm3 | Boiling Point | 789.9ºC at 760mmHg |
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| Molecular Formula | C30H32N2O12 | Melting Point | / |
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| MSDS | ChineseUSA | Flash Point | 431.5ºC |
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Names
| Name | acetyloxymethyl 2-[4-[bis[2-oxo-2-(2-oxopropoxymethoxy)ethyl]amino]phenyl]-1H-indole-6-carboxylate |
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| Synonym | More Synonyms |
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Mag-indo-1/AM BiologicalActivity
| Description | Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2]. |
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| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
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| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Measurement of intracellular Ca2+[1]: 1. Incubate 3 mL cell suspension with 10 µM Mag-indo-1/AM for 15 min at 30℃. 2. Centrifuge the cells for 1 min at 500 rpm and resuspend in 5 mL isolation buffer (containing 1 mM CaCl2). 3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol and store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use. 4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend and store at room temperature. 6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope. 7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm (the peak emissions of the Ca2+ bound and Ca2+ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system. |
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| References | [1]. Griffiths EJ. Calcium handling and cell contraction in rat cardiomyocytes depleted of intracellular magnesium. Cardiovasc Res. 2000 Jul;47(1):116-23. [2]. Morelle B, et al. Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1. Anal Biochem. 1994 Apr;218(1):170-6. |
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Chemical & Physical Properties
| Density | 1.335g/cm3 |
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| Boiling Point | 789.9ºC at 760mmHg |
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| Molecular Formula | C30H32N2O12 |
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| Molecular Weight | 612.58100 |
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| Flash Point | 431.5ºC |
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| Exact Mass | 612.19600 |
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| PSA | 176.83000 |
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| LogP | 2.53130 |
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| Vapour Pressure | 6.32E-25mmHg at 25°C |
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| Index of Refraction | 1.585 |
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| InChIKey | XLQNJLJDRRJLCB-UHFFFAOYSA-N |
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| SMILES | CC(=O)COCOC(=O)CN(CC(=O)OCOCC(C)=O)c1ccc(-c2cc3ccc(C(=O)OCOC(C)=O)cc3[nH]2)cc1 |
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Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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| RIDADR | NONH for all modes of transport |
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Synonyms
| Indo 1-AM analog |
| Mag-Indo 1-AM |
| Mag-Indo-1AM |
