Introduction：Basic information about CAS 313967-18-9|FLI-06, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | FLI-06 |
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| CAS Number | 313967-18-9 | Molecular Weight | 438.516 |
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| Density | 1.3±0.1 g/cm3 | Boiling Point | 597.5±50.0 °C at 760 mmHg |
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| Molecular Formula | C25H30N2O5 | Melting Point | / |
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| MSDS | USA | Flash Point | 315.2±30.1 °C |
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Names
| Name | 3-Quinolinecarboxylic acid, 1,4,5,6,7,8-hexahydro-2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-, cyclohexyl ester |
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| Synonym | More Synonyms |
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FLI-06 BiologicalActivity
| Description | FLI-06 is an inhibitor of Notch signaling with an EC50 of 2.3 μM. |
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| Related Catalog | Signaling Pathways >>Stem Cell/Wnt >>NotchResearch Areas >>Cancer |
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| Target | EC50: 2.3 μM (Notch signaling)[1] |
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| In Vitro | FLI-06, disrupted the Golgi apparatus in a manner distinct from that of brefeldin A and golgicide A. FLI-06 inhibited general secretion at a step before exit from the endoplasmic reticulum. In FLI-06–treated cells, no APPCTF accumulates despite strongly reduced Aβ secretion, suggesting that it acts upstream of α-secretase and β-secretase cleavage. FLI-06 is a very useful chemical probe to study the inhibition of membrane traffic at pre- ER-exit site (ERES) stages without fusion of ER-Golgi[1]. |
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| Kinase Assay | EC50 values of the test compounds are calculated from serial dilution series ranging from 200 to 0.1 μM. Cells are seeded in 96-well plates at a density of 5,000 cells per well in 100 μL medium. The next day, 100 μL medium containing each test compound is added. Cells are incubated for 16 h, fixed and processed for automated microscopy. EC50 estimates are calculated using four-parameter log-logistic fit with the package drc[1]. |
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| References | [1]. Kramer A, et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat Chem Biol. 2013 Nov;9(11):731-8. |
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Chemical & Physical Properties
| Density | 1.3±0.1 g/cm3 |
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| Boiling Point | 597.5±50.0 °C at 760 mmHg |
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| Molecular Formula | C25H30N2O5 |
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| Molecular Weight | 438.516 |
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| Flash Point | 315.2±30.1 °C |
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| Exact Mass | 438.215485 |
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| PSA | 101.22000 |
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| LogP | 4.47 |
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| Appearance of Characters | white to beige |
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| Vapour Pressure | 0.0±1.7 mmHg at 25°C |
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| Index of Refraction | 1.596 |
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| InChIKey | SWWVFYHSSOWZMF-UHFFFAOYSA-N |
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| SMILES | CC1=C(C(=O)OC2CCCCC2)C(c2ccc([N+](=O)[O-])cc2)C2=C(CC(C)(C)CC2=O)N1 |
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| Storage condition | 2-8°C |
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| Water Solubility | DMSO: soluble5mg/mL, clear (warmed) |
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Safety Information
Synonyms
| Cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydro-3-quinolinecarboxylate |
| FLI-06 |
| 1,4,5,6,7,8-Hexahydro-2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-3-quinolinecarboxylic acid cyclohexyl ester |
