Introduction：Basic information about CAS 166090-74-0|Amyloid β-Protein (1-42) (mouse, rat), including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Amyloid β-Protein (1-42) (mouse, rat) |
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| CAS Number | 166090-74-0 | Molecular Weight | 4418.017 |
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| Density | / | Boiling Point | / |
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| Molecular Formula | C199H307N53O59S | Melting Point | / |
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| MSDS | / | Flash Point | / |
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Names
| Name | Amyloid beta-peptide (1-42) (rat) |
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| Synonym | More Synonyms |
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Amyloid β-Protein (1-42) (mouse, rat) BiologicalActivity
| Description | β-Amyloid (1-42), rat is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease. |
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| Related Catalog | Signaling Pathways >>Neuronal Signaling >>Amyloid-βPeptidesResearch Areas >>Neurological Disease |
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| Target | Amyloid-β[1] |
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| In Vitro | β-Amyloid (1-42), rat shows cytotoxic effect on the hippocampal slices at 20 μM[1]. β-Amyloid (1-42), rat causes morphological changes in NGF-induced PC12 cells, induces formed cell processes to retract in differentiated cells and affect the expression of exons 2/3 in both undifferentiated and differentiated cells[2]. |
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| Cell Assay | After treating the hippocampal slices with 20 μM β-Amyloid (1-42) for 4 h, supernatant is replaced by fresh H-ASCF/3 (0.9 mL/chamber) to which 0.1 mL MTT stock solution (5 mg/mL H-ACSF/3) is added (MTT final concentration: 0.5 mg/mL). The chamber is left to rest for 15 min without carboxygenation. To stop further reduction of MTT, the medium (H-ACSF/3) is removed. The slices are transferred into 96-well plate, then pure DMSO (100 μL/slice/well) is added for dissolving formazane from the slices. (30 min in a 96-well plate). Then 70 μL DMSO solution from each slice (well) is transferred into another 96-well plate. The optical density (OD) of the dissolved formazane is measured at 550 and 620 nm[1]. |
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| References | [1]. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5. [2]. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34. |
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Chemical & Physical Properties
| Molecular Formula | C199H307N53O59S |
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| Molecular Weight | 4418.017 |
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| Exact Mass | 4417.244 |
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| InChIKey | HAWSUONKNKRLRH-ANECVMDESA-N |
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| SMILES | CCC(C)C(NC(=O)C(C)NC(=O)CNC(=O)C(CCCCN)NC(=O)C(CC(N)=O)NC(=O)C(CO)NC(=O)CNC(=O)C(NC(=O)C(CC(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(C)NC(=O)C(Cc1ccccc1)NC(=O)C(Cc1ccccc1)NC(=O)C(NC(=O)C(CC(C)C)NC(=O)C(CCCCN)NC(=O)C(CCC(N)=O)NC(=O)C(Cc1c[nH]cn1)NC(=O)C(CCCNC(=N)N)NC(=O)C(NC(=O)C(CCC(=O)O)NC(=O)C(Cc1ccccc1)NC(=O)CNC(=O)C(CO)NC(=O)C(CC(=O)O)NC(=O)C(Cc1c[nH]cn1)NC(=O)CNC(=O)C(Cc1ccccc1)NC(=O)C(CCC(=O)O)NC(=O)C(C)NC(=O)C(N)CC(=O)O)C(C)C)C(C)C)C(C)C)C(=O)NC(C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CCSC)C(=O)NC(C(=O)NCC(=O)NCC(=O)NC(C(=O)NC(C(=O)NC(C(=O)NC(C)C(=O)O)C(C)CC)C(C)C)C(C)C)C(C)C)C(C)CC |
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Synonyms
| bap 1-42 |
| asp-ala-glu-phe-arg-his-asp-ser-gly-tyr-glu-val-his-his-gln-lys-leu-val-phe-phe-ala-glu-asp-val-gly-ser-asn-lys-gly-ala-ile-ile-gly-leu-met-val-gly-gly-val-val-ile-ala |
| predominant amyloid b-protein fragment |
| beta-amyloid peptide (1-40), mouse, rat |
| asp-ala-glu-phe-gly-his-asp-ser-gly-phe-glu-val-arg-his-gln-lys-leu-val-phe-phe-ala-glu-asp-val-gly-ser-asn-lys-gly-ala-ile-ile-gly-leu-met-val-gly-gly-val-val-ile-ala |
| beta-amyloid peptide (1-40), rat |
| beta-amyloid, sodium salt |
| beta-amyloid peptide (1-42), rat |
| β-Amyloid (1-42), rat |
