CAS 31852-29-6|Polyinosinic-polycytidylic acid potassium
| Common Name | Polyinosinic-polycytidylic acid potassium | ||
|---|---|---|---|
| CAS Number | 31852-29-6 | Molecular Weight | 710.5 |
| Density | / | Boiling Point | / |
| Molecular Formula | (C10H13N4O8P)x.(C9H14N3O8P)x.xK | Melting Point | / |
| MSDS | USA | Flash Point | / |
Names
| Name | POLYINOSINIC-POLYCYTIDYLIC ACID POTASSIUM SALT |
|---|---|
| Synonym | More Synonyms |
BiologicalActivity
| Description | Polyinosinic-polycytidylic acid potassium (Poly(I:C) potassium) is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5). Polyinosinic-polycytidylic acid sodium can be used as a vaccine adjuvant to enhance innate and adaptive immune responses, and to alter the tumor microenvironment. Polyinosinic-polycytidylic acid potassium can directly trigger cancer cells to undergoApoptosis[1][2][3]. |
|---|---|
| Related Catalog | Signaling Pathways >>Immunology/Inflammation >>Toll-like Receptor (TLR)Signaling Pathways >>Apoptosis >>ApoptosisResearch Areas >>CancerResearch Areas >>Inflammation/Immunology |
| Target | TLR3 |
| In Vitro | Polyinosinic-polycytidylic acid potassium (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) 抑制细胞生长活性[1]. Polyinosinic-polycytidylic acid potassium (200 ng/mL; 24 hours; 1205Lu cells) 诱导 1205Lu 细胞凋亡[1]。 Polyinosinic-polycytidylic acid potassium (5 ng/mL; 24 hours; 1205Lu cells) 诱导黑素瘤细胞中的 IFN-β 表达。RIG-I 和 MDA-5 的沉默证实 Polyinosinic-polycytidylic acid potassium 诱导 IFN-β 分别需要 RIG-I 和 MDA-5，并且需要 IPS-1[1]。 Polyinosinic-polycytidylic acid potassium 重悬于无菌盐水中，以 2 mg/ml 的浓度加热至 50 °C 以确保完全溶解，然后自然冷却至室温以确保双链 RNA 的适当退火，从而制备用于注射的 Polyinosinic-polycytidylic acid potassium. Poly I:C 储存在 -20 °C 直至使用[3]。 Cell Viability Assay[1] Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells Concentration: 20 ng/mL Incubation Time: 24 hours Result: Strongly reduced viability from 100% in controls to 20%–50% within 24 hours. Apoptosis Analysis[1] Cell Line: 1205Lu cells Concentration: 200 ng/mL Incubation Time: 24 hours Result: Induced apoptosis in 1205Lu cells. RT-PCR[1] Cell Line: WM793, WM278, WM239A, WM9 and 1205Lu cells Concentration: 3 ng/mL Incubation Time: 24 hours Result: Induced IFN-β expression in melanoma cells. Western Blot Analysis[1] Cell Line: 1205Lu cells Concentration: 5 ng/mL Incubation Time: 24 hours Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells. |
| In Vivo | Polyinosinic-polycytidylic acid potassium (50 µg; i.v.; on days 3, 6, and 9 after tumor inoculation) 在 NOD/SCID 免疫缺陷小鼠中显示出抗肿瘤活性[1]。 Animal Model: NOD/SCID immunodeficient mice (1205Lu cells)[1] Dosage: 50 µg Administration: I.v.; on days 3, 6, and 9 after tumor inoculation Result: Reduced the size of metastases and the total amount of tumor tissue, and the level of human DNA was 50% lower in mice. |
| References | [1]. Besch R, et al. Proapoptotic signaling induced by RIG-I and MDA-5 results in type I interferon-independent apoptosis in human melanoma cells. J Clin Invest. 2009 Aug;119(8):2399-411. [2]. Cheng YS, et al. Anticancer function of polyinosinic-polycytidylic acid. Cancer Biol Ther. 2010 Dec 15;10(12):1219-23. [3]. Robert Field, et al. Systemic challenge with the TLR3 agonist poly I:C induces amplified IFNalpha/beta and IL-1beta responses in the diseased brain and exacerbates chronic neurodegeneration. Brain Behav Immun. 2010 Aug;24(6):996-1007. |
Chemical & Physical Properties
| Molecular Formula | (C10H13N4O8P)x.(C9H14N3O8P)x.xK |
|---|---|
| Molecular Weight | 710.5 |
| InChIKey | KHPUABRWHJGXNJ-VRQAYDGLSA-N |
| SMILES | Nc1ccn(C2OC(COP(=O)(O)O)C(O)C2O)c(=O)n1.O=c1[nH]cnc2c1ncn2C1OC(COP(=O)(O)O)C(O)C1O.[K] |
| Storage condition | −20°C |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xi |
| RIDADR | NONH for all modes of transport |
Articles11
More Articles| The interaction between maternal immune activation and alpha 7 nicotinic acetylcholine receptor in regulating behaviors in the offspring. Brain. Behav. Immun. 46 , 192-202, (2015) Mutation of human chromosome 15q13.3 increases the risk for autism and schizophrenia. One of the noteworthy genes in 15q13.3 is CHRNA7, which encodes the nicotinic acetylcholine receptor alpha 7 subun... | |
| How reliable are sino-nasal cell lines for studying the pathophysiology of chronic rhinosinusitis? Ann. Otol. Rhinol. Laryngol. 124 , 437-42, (2015) Well-characterized cell lines represent useful scientific tools to study the pathophysiology of human disease. Chronic rhinosinusitis (CRS) is a very common condition, though the number of CRS cell li... | |
| RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality. Proc. Natl. Acad. Sci. U. S. A. 112 , 11007-12, (2015) Systematic inflammation contributes to the development of many diseases, including cardiovascular disease, which is the leading cause of mortality worldwide. How such inflammation is initiated and mai... |
Synonyms
| MFCD00131983 |
