Chloroquine diphosphate CAS 50-63-5

Introduction：Basic information about Chloroquine diphosphate CAS 50-63-5, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Chloroquine diphosphate Basic information

• Product Name: Chloroquine diphosphate
• Synonyms: 7-chlor-4-(4-(diaethylamino)-1-methylbutylamino)-chinolindiphosphat;ARALEN PHOSPHATE;CHLOROQUINE DIPHOSPHATE;CHLOROQUINE DIPHOSPHATE SALT;CHLOROQUINE PHOSPHATE;7-CHLORO-4-OXO-1H-QUINOLINE-3-CARBOXYLIC ACID;CHLOROQUINE PHOSPHATE BP;CHLOROQUINE PHOSPHATE BP98
• CAS: 50-63-5
• MF: C18H32ClN3O8P2
• MW: 515.86
• EINECS: 200-055-2
• Product Categories: Inhibitors;ARALEN;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Miscellaneous Enzyme;API;50-63-5;Coronavirus
• Mol File: 50-63-5.mol

Chloroquine diphosphate Chemical Properties

• Melting point: 200 °C (dec.) (lit.)
• vapor pressure: <0.0000001 kPa ( 25 °C)
• storage temp.: protect from light
• solubility: H₂O: 50 mg/mL, clear
• pka: pKa 8.10(H2Ot = 20c = 0.0025) (Uncertain)
• form: solid
• color: White
• PH: pH(100g/l, 25℃) : 3.8～4.3
• biological source: synthetic
• Water Solubility: Soluble in water
• Merck: 14,2163
• BRN: 4223142
• BCS Class: 1
• Stability: Stable for 2 years from date of purchase as supplied. Solutions in distilled water may be stored at -20° for up to 3 months.
• InChI: 1S/C18H26ClN3.2H3O4P/c1-4-22(5-2)12-6-7-14(3)21-17-10-11-20-18-13-15(19)8-9-16(17)18;2*1-5(2,3)4/h8-11,13-14H,4-7,12H2,1-3H3,(H,20,21);2*(H3,1,2,3,4)
• InChIKey: QKICWELGRMTQCR-UHFFFAOYSA-N
• SMILES: OP(O)(O)=O.OP(O)(O)=O.CCN(CC)CCCC(C)Nc1ccnc2cc(Cl)ccc12
• CAS DataBase Reference: 50-63-5(CAS DataBase Reference)
• EPA Substance Registry System: Chloroquine diphosphate (50-63-5)

Safety Information

• Hazard Codes: Xn
• Risk Statements: 22-40-20/21/22
• Safety Statements: 22-24/25-36
• RIDADR: 1544
• WGK Germany: 3
• RTECS: VB2450000
• F: 10
• HazardClass: 6.1(b)
• PackingGroup: III
• HS Code: 2933492250
• Storage Class: 11 - Combustible Solids
• Hazard Classifications: Acute Tox. 4 Oral
• Toxicity: LD50 oral in rat: 623mg/kg

Chloroquine diphosphate Usage And Synthesis

Description

Chloroquine is an aminoquinoline that is an inhibitor of autophagy and has antimalarial, anti-inflammatory, anticancer, and antiviral activities. Chloroquine inhibits autophagosome-lysosome fusion in HeLa cells when used at a concentration of 100 μM. It is active against the chloroquine-sensitive GC03 strain of P. falciparum (IC₅₀ = 29.2 nM) but has decreased activity against mutant pfcrt P. falciparum (IC₅₀s = 100-150 nM). Chloroquine prevents infection by severe acute respiratory coronavirus 2 (SARS-CoV-2) in Vero cells (EC₅₀ = 1.13 μM) but does not inhibit SARS-CoV replication in the lungs in a mouse model of SARS-CoV infection. It inhibits the growth of human SSC25 and CAL 27 oral squamous cell carcinoma cells (IC₅₀s = 29.9 and 17.3 μM, respectively), as well as A498, SN12C, RXF 393, and 769-P renal cancer cells (IC₅₀s = 16, 62, 81, and 25 μM, respectively). It reduces tumor growth in a CAL 27 mouse xenograft model and a 4T1 mouse allograft model when administered at a dose of 50 mg/kg. Formulations containing chloroquine have been used in the prevention of malaria, as well as the treatment of rheumatoid arthritis and systemic lupus erythematosus (SLE), and have been associated with cardiotoxicity and myopathy.

Chemical Properties

White Solid

Originator

Nivaquine,Specia,France,1949

Uses

Chloroquine diphosphate salt is used to study the role of endosomal acidification in cellular processes, such as the signaling of intracellular TLRs. It can be used as DNA intercalator & to dissociate antigen antibody complexes without denaturing red blood cell antigens. Chloroquine showed very high antiviral activity against NiV but very little activity against the other viruses at concentrations lower than 20 μM.

Uses

Standard anti-malarial drug. Substrate for MRP in multidrug resistant cell line and inhibits photoaffinity labelling of MRP by quinoline-based photoactive drug IAAQ

Uses

antimalarial, antiamebic, antirheumatic, intercalating agent

Uses

An antimalarial compound

Manufacturing Process

105 g of 4,7-dichloroquinoline (MP 93 to 94°C) are heated with 200 g of 1-diethylamino-4-aminopentane for 7 hours in an oil bath to 180°C whilestirring, until a test portion dissolved in diluted nitric acid does not show aprecipitation with sodium acetate solution. The mixture is dissolved in dilutedacetic acid and made alkaline by adding sodium lye.
The base is extracted with ether, dried with potassium carbonate, the etherremoved by distillation and the residue fractionated. The 4-(5'-diethylaminopentyl-2'-amino)-7-chloroquinoline (BP 212 to 214C/0.2 mm) isobtained. On cooling the compound solidifies crystalline. It melts,recrystallized from benzene, at 88°C. The base combines with phosphoric acidto yield a diphosphate salt.

Therapeutic Function

Antimalarial

General Description

Chloroquine phosphate is a phosphate salt of chloroquine. It belongs to the class of aminoquinoline drugs utilized as antimalarials and amebicides.

Biochem/physiol Actions

Standard anti-malarial drug. Substrate for MRP in multidrug resistant cell line and inhibits photoaffinity labeling of MRP by quinoline-based photoactive drug IAAQ (N-[4-[1-hydroxy-2-(dibutylamino)ethyl]quinolin-8-yl]-4-azidosalicylamide).

storage

Desiccate at RT

References

[1] HERMANN B FRIEBOES. Chloroquine-mediated cell death in metastatic pancreatic adenocarcinoma through inhibition of autophagy.[J]. Journal of the Pancreas, 2014, 15 2: 189-197. DOI:10.6092/1590-8577/1900
[2] PEI-DU JIANG. Antitumor and antimetastatic activities of chloroquine diphosphate in a murine model of breast cancer[J]. Biomedicine & Pharmacotherapy, 2010, 64 9: Pages 609-614. DOI:10.1016/j.biopha.2010.06.004
[3] DONG SOON CHOI. Chloroquine Eliminates Cancer Stem Cells Through Deregulation of Jak2 and DNMT1[J]. STEM CELLS, 2014, 32 9: 2309-2323. DOI:10.1002/stem.1746
[4] JEAN M MULCAHY LEVY. Autophagy inhibition improves chemosensitivity in BRAF(V600E) brain tumors.[J]. Cancer discovery, 2014, 4 7: 773-780. DOI:10.1158/2159-8290.cd-14-0049

Chloroquine diphosphate Preparation Products And Raw materials

• Raw materials: 2-Amino-5-diethylaminopentane-->4,7-Dichloroquinoline-->Phosphoric acid