MEDROXYPROGESTERONE CAS 520-85-4

Introduction：Basic information about MEDROXYPROGESTERONE CAS 520-85-4, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

MEDROXYPROGESTERONE Basic information

• Product Name: MEDROXYPROGESTERONE
• Synonyms: PREGN-4-ENE-3,20-DIONE,17-HYDROXY-6-ALPHA-METHYL-;17α-Hydroxy-6α-methyl-4-pregnene-3,20-dione, 17α-Hydroxy-6α-methylprogesterone, 6α-Methyl-5-pregnen-17α-ol-3,20-dione, 6-Dihydromegestrol;(6S,8R,9S,10R,13S,14S,17R)-17-acetyl-17-hydroxy-6,10,13-trimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one;(6S,8R,9S,10R,13S,14S,17R)-17-ethanoyl-17-hydroxy-6,10,13-trimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one;4-Pregnen-6a-Methyl-17-ol-3,20-dione-d7;(6S,8R,9S,10R,13S,14S,17R)-17-acetyl-17-hydroxy-6,10,13-triMethyl-6,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3(2H)-one;Medroxyprogesterone acetate-13C2-d3;6alpha-Methyl-17alpha-hydroxypregn-4-ene-3,20-dione
• CAS: 520-85-4
• MF: C22H32O3
• MW: 344.49
• EINECS: 208-298-6
• Product Categories: Intermediates & Fine Chemicals;Isotope Labelled Compounds;Pharmaceuticals;Steroids;Hormone Drugs
• Mol File: 520-85-4.mol

MEDROXYPROGESTERONE Chemical Properties

• Melting point: 204-206°C
• alpha: D25 +75° (in chloroform)
• Boiling point: 419.57°C (rough estimate)
• density: 1.0906 (rough estimate)
• refractive index: 1.5000 (estimate)
• storage temp.: Sealed in dry,Room Temperature
• solubility: Chloroform (Slightly), Methanol (Slightly)
• form: Solid
• pka: 13.03±0.70(Predicted)
• color: White
• Merck: 13,5817
• BRN: 2510965
• InChI: 1S/C22H32O3/c1-13-11-16-17(20(3)8-5-15(24)12-19(13)20)6-9-21(4)18(16)7-10-22(21,25)14(2)23/h12-13,16-18,25H,5-11H2,1-4H3/t13-,16+,17-,18-,20+,21-,22-/m0/s1
• InChIKey: FRQMUZJSZHZSGN-HBNHAYAOSA-N
• SMILES: C1[C@@]2(C)C([C@H](C[C@]3([H])[C@]2([H])CC[C@@]2(C)[C@@]3([H])CC[C@@]2(C(C)=O)O)C)=CC(=O)C1
• CAS DataBase Reference: 520-85-4(CAS DataBase Reference)
• EPA Substance Registry System: Medroxyprogesterone (520-85-4)

Safety Information

• Hazard Codes: Xn
• Risk Statements: 48-63-68
• Safety Statements: 22-24/25
• WGK Germany: 3
• RTECS: TU5300000
• HS Code: 29372390
• Storage Class: 11 - Combustible Solids
• Hazard Classifications: Repr. 2
• Hazardous Substances Data: 520-85-4(Hazardous Substances Data)

MEDROXYPROGESTERONE Usage And Synthesis

Chemical Properties

MEDROXYPROGESTERONE is White Solid

Originator

Provera,Upjohn,US,1959

Uses

An orally active progestogen used in hormone replacement therepy (HRT), in the past has been used as a component of oral contraceptives.SMedroxyprogesterone Acetate USP Related Compound B.

Uses

MEDROXYPROGESTERONE is an orally active progestogen used in hormone replacement therepy (HRT), in the past has been used as a component of oral contraceptives.

Definition

ChEBI: A 3-oxo Delta4-steroid that is pregn-4-ene-3,20-dione substituted by an alpha-hydroxy group at position 17 and a methyl group at position 6.

Manufacturing Process

Preparation of 17α-Hydroxyprogesterone 3,20-Bis-(Ethylene Ketal): A solution was prepared containing 50.0 g of 17α-hydroxyprogesterone in 1,000 ml of benzene, 100 ml of ethylene glycol and 2.5 g of p-toluenesulfonic acid monohydrate. This mixture was refluxed for a period of 17 hours using a calcium carbide water-trap to remove the water formed in the reaction. After this period of reflux 6.5 ml of pyridine was added to the solution, and the mixture cooled to room temperature.
The lower glycol layer was separated and washed with benzene. The benzenelayer and the benzene washings were combined and the combined solutionwas divided into two equal portions, one of which was used for the isolation of17α-hydroxyprogesterone 3,20-bis-(ethylene ketal) as follows. The benzenesolution was washed with 5% sodium carbonate solution, water and saturated sodium chloride solution. After being dried over anhydrous magnesium sulfatethe solution was concentrated to dryness at reduced pressure, The residuewas recrystallized by taking up in hot methylene chloride, adding acetone andboiling to remove the methylene chloride until a final volume of about 200 mlwas reached.
The solution was then refrigerated overnight and 17.8 g of crystals wereremoved by filtration. A second crop was obtained yielding 3.7 g ofcompound. The total yield of 17α-hydroxyprogesterone 3,20-bis-(ethyleneketal) was 20.3 g (64.3% of theory). Recrystallization of the crude 17αhydroxyprogesterone 3,20-bis-(ethylene ketal) from methanol gave the purebisketal of MP 209° to 211°C.
Preparation of 5α,6α-Oxido-17α-Hydroxyallopregnane-3,20-dione 3,20-Bis-(Ethylene Ketal): A solution was prepared by heating 19.96 g (0.0477 mol) of17α-hydroxyprogesterone 3,20-bis-(ethylene ketal) and 500 ml of benzene.After the solution was effected the flask was cooled to 5°C and a mixture of3.68 g (0.0449 mol) of sodium acetate and 174 ml of 40% peracetic acid wasadded with stirring. The reaction mixture was stirred in the ice bath for 3hours. The lower peracid layer was separated, diluted with water andextracted twice with benzene.
The upper layer was neutralized by the addition of cold 10% sodium hydroxidesolution while stirring in an ice bath. The rate of addition of the sodiumhydroxide was regulated to keep the temperature below 10°C. The benzeneextracts from the peracid layer were combined and washed with cold 10%sodium hydroxide solution and with saturated sodium chloride solution. All theaqueous layers were washed again with the same portion of benzene. Thecombined benzene layers were dried over anhydrous magnesium sulfate andconcentrated to dryness at reduced pressure.
The residue was recrystallized from acetone using methylene chloride to aid insolution. The crystalline material was removed by filtration and wasrecrystallized from methylene chloride-acetone to yield a total of 8 g of 5α,6αoxido-17α-hydroxyallopregnane-3,20-dione 3,20-bis-(ethylene ketal) of MP211° to 215°C. For analytical purposes, another recrystallization frommethylene chloride-acetone gave pure 5α,6α-oxido-17α-hydroxyallopregnane3,20-dione 3,20-bis-(ethylene ketal) of MP 216° to 218.5°C.
Preparation of 5α,17α-Dihydroxy-6β-Methylallopregnane-3,20-dione 3,20-bis-(Ethylene Ketal): To a solution of 91.6 g of 5α,6α-oxido-17αhydroxyallopregnane-3,20-dione 3,20-bis-(ethylene ketal) in 3,500 ml offreshly distilled tetrahydrofuran was added 1,170 ml of commercial 3 molarmethyl magnesium bromide in ether solution. The reaction mixture was boiledto remove 1,800 ml of solvent by distillation and thereafter 1,000 ml offreshly distilled tetrahydrofuran was added.
Boiling was continued under reflux for a period of 16 hours. The solution wasthen concentrated to about one-half its original volume by distillation and waspoured slowly with vigorous stirring into a large volume of ice watercontaining 340 g of ammonium chloride. The aqueous solution was saturatedwith sodium chloride and extracted with benzene. The benzene extract waswashed with saturated brine, and both aqueous layers were washed againwith the same portions of benzene.
The combined benzene layers were dried over anhydrous sodium carbonateand the solvent was removed at reduced pressure to give 90.5 g of crudecrystalline 5α,17α-dihydroxy-6β-methylallopregnane-3,20-dione 3,20-bis-(ethylene ketal). Half of the residue, 45.2 g, was recrystallized from acetoneand some methylene chloride to give 34.4 g of 5α,17α-dihydroxy-6βmethylallopregnane-3,20-dione 3,20-bis-(ethylene ketal). A samplerecrystallized from acetone and methylene chloride for analysis melted at160° to 163°C.
Preparation of 5α,17α-Dihydroxy-6β-Methylallopregnane-3,20-dione: Asolution was prepared containing 38.9 g of 5α,7α-dihydroxy-6βmethylallopregnane-3,20-dione 3,20-bis-(ethylene ketal) in 389 ml of boilingacetone. Thereto was added 39 ml of 1 N sulfuric acid in portions underswirling and seeding with product. Boiling was continued for a period of 2minutes and the mixture was allowed to stand at room temperature.Thereafter the mixture was diluted with 1,500 ml of water, chilled and filtered.
The precipitate was washed with water, dilute ammonium hydroxide andwater, and dried in a vacuum oven overnight. The yield was 31.2 g which wasrecrystallized by dissolving in 1,200 ml of dimethylformamide, heating to150°C, cooling slightly, and adding 12 ml of hot water. The recrystallized5α,17α-dihydroxy-6β-methylallopregnane-3,20-dione thus obtained was 28.75g of MP 270° to 275.5°C. After an additional recrystallization from aqueousdimethylformamide, the MP was 274° to 279°C.
Preparation of 6α-Methyl-17α-Hydroxyprogesterone: A suspension was madeby introducing 2 g of 5α,17α-dihydroxy-6β-methylallopregnane-3,20-dioneinto 200 ml of chloroform. The suspension was chilled in an ice bath withstirring, and thereupon hydrogen chloride was bubbled through the reactionmixture for 80 minutes with continuous cooling and stirring. After bubbling innitrogen for a period of 15 minutes the solution was washed with water, 1 Nsodium bicarbonate solution and again with water.
The aqueous layers were rewashed with one portion of chloroform, and thewashings combined with the remainder of the chloroform solution. Afterdrying over anhydrous magnesium sulfate, the chloroform solution wasconcentrated to dryness, then taken up in a small volume of methylenechloride, treated with Magnesol anhydrous magnesium silicate and filtered.Acetone was added to the solution and the solution was boiled to remove themethylene chloride. After the solution was concentrated to a volume of about15 ml it was chilled and the crystals were collected through filtration. The1.37 g of crystals so obtained were recrystallized from acetone to give pure6α-methyl-17α-hydroxyprogesterone of MP 220° to 223.5°C.
Preparation of 6α-Methyl-17-Hydroxyprogesterone 17-Acetate: 1 g of 6αmethyl-17α-hydroxyprogesterone was dissolved in a mixture of 10 ml of aceticacid and 2 ml of acetic anhydride by heating. After solution was effected themixture was cooled to 15°C, and 0.3 g of p-toluenesulfonic acid was added.After allowing the mixture to stand for a period of 2.5 hours at roomtemperature, the pink solution was poured into ice water to give anamorphous solid which was recovered by filtration.
The precipitate was washed carefully with water and was then dissolved in 10ml of methanol and 1.5 ml of methylene chloride. The solution was concentrated to 10 ml, diluted with 0.5 ml of 10% sodium hydroxide, boiledfor one minute and cooled. The product, which crystallized on cooling, wasrecrystallized to give flakes of 6α-methyl-17α-hydroxyprogesterone 17-acetate, having a MP 205° to 209°C, according to US Patent 3,147,290.

Brand name

Amen (Amarin);Curretab (Solvay Pharmaceuticals); Cycrin (ESI); Provera(Pharmacia & Upjohn).

Therapeutic Function

Progestin

Purification Methods

If it contains the epi-isomer (TLC), then dissolve it in CHCl3, bubble dry HCl gas to epimerise it, evaporate and recrystallise it from chloroform. The UV has max at 241nm ( 16,150) in EtOH. The 17-acetate [71-58-9] crystallises from MeOH with m 207-208o and [] D 25 +61o (CHCl3). Its UV has max at 240nm ( 15,950) in EtOH. [Babcock et al. J Am Chem Soc 80 2904 1958, Beilstein 8 IV 2212.]

MEDROXYPROGESTERONE Preparation Products And Raw materials

• Raw materials: Acetic anhydride-->Peroxyacetic acid-->Sulfuric acid-->Methylmagnesium Bromide-->Ethylene glycol-->Pregnane-3,20-dione, 5,17-dihydroxy-6-methyl-, (5α,6β)--->6β-Medroxyprogesterone-->3-beta,17-alpha-dihydroxypregn-5-en-20-one 3-acetate-->6-METHYL-17A-HYDROXY PREGNENOLONE DIACETATE)-->Medroxyprogesterone Acetate-->17-hydroxypregn-5-ene-3,20-dione cyclic bis(ethylene acetal)-->(5α,6α)-Epoxy-17α-hydroxy-pregnane-3,20-dione-3,20-bis(ethyleneketal)