Introduction：Basic information about CAS 1628208-23-0|CPI-455, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | CPI-455 |
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| CAS Number | 1628208-23-0 | Molecular Weight | 278.309 |
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| Density | 1.3±0.1 g/cm3 | Boiling Point | 488.7±55.0 °C at 760 mmHg |
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| Molecular Formula | C16H14N4O | Melting Point | / |
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| MSDS | / | Flash Point | 249.3±31.5 °C |
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Names
| Name | 6-Isopropyl-7-oxo-5-phenyl-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile |
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| Synonym | More Synonyms |
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CPI-455 BiologicalActivity
| Description | CPI-455 is a specific KDM5 inhibitor. |
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| Related Catalog | Signaling Pathways >>Epigenetics >>Histone DemethylaseResearch Areas >>Cancer |
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| Target | KDM5[1] |
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| In Vitro | CPI-455 mediates KDM5 inhibition, elevates global levels of H3K4 trimethylation (H3K4me3) and decreases the number of DTPs in multiple cancer cell line models treated with standard chemotherapy or targeted agents[1]. CPI-455, with high measured affinity for the target KDM5 proteins. Within 24 hours, increases in H3K4me3, but not H3K4me2, are observed after exposure to either of the two active compounds, CPI-455 and CPI-766, in a dosedependent manner. IC50 calculation for KDM5 Inhibitor CPI0455 in 3 luminal breast cancer cell lines MCF-7, T-47 and EFM-19 are 35.4, 26.19 and 16.13 μM, respectively[2]. |
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| Cell Assay | MCF-7 is a luminal breast cancer cell line. MCF-7 is grown in MEM supplemented with 10% fetal bovine serum (FBS) and is incubated at 37°C with a 5% CO2 atmosphere. For each treatment condition, 2.5×105 MCF-7 cells are plated in T25 flask and allowed to rest overnight. The following day, the media is aspirated and replaced with media containing DAC at a final concentration of 62.5 nM. DAC media is aspirated and replaced every 24 hours for a total treatment time of 72 hours. Following DAC treatment, media is aspirated and replaced with media supplemented with 2% FBS containing KDM5 inhibitors (e.g., CPI-455) at a final concentration of 9.375 μM. Cells are harvested following 72 hours of KDM5 inhibitor treatment[1]. |
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| References | [1]. Vinogradova M, et al. An inhibitor of KDM5 demethylases reduces survival of drug-tolerant cancer cells. Nat Chem Biol. 2016 Jul;12(7):531-8. [2]. Benjamin R. Leadem. NOVEL HISTONE DEMETHYLASE INHIBITORS SYNERGISTICALLY |
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Chemical & Physical Properties
| Density | 1.3±0.1 g/cm3 |
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| Boiling Point | 488.7±55.0 °C at 760 mmHg |
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| Molecular Formula | C16H14N4O |
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| Molecular Weight | 278.309 |
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| Flash Point | 249.3±31.5 °C |
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| Exact Mass | 278.116760 |
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| LogP | 2.46 |
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| Appearance of Characters | white solid |
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| Vapour Pressure | 0.0±1.2 mmHg at 25°C |
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| Index of Refraction | 1.670 |
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| InChIKey | VGXRQCOVGLGFIM-UHFFFAOYSA-N |
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| SMILES | CC(C)c1c(-c2ccccc2)nc2c(C#N)c[nH]n2c1=O |
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| Storage condition | -20℃ |
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Synonyms
| 6-Isopropyl-7-oxo-5-phenyl-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile |
| Pyrazolo[1,5-a]pyrimidine-3-carbonitrile, 4,7-dihydro-6-(1-methylethyl)-7-oxo-5-phenyl- |
| CPI-455 |
