5(6)-Carboxyfluorescein CAS 72088-94-9
Introduction：Basic information about 5(6)-Carboxyfluorescein CAS 72088-94-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
5(6)-Carboxyfluorescein Basic information
| Product Name: | 5(6)-Carboxyfluorescein |
| Synonyms: | 9’-[9h]xanthene]-ar-carboxylicacid,3’,6’-dihydroxy-spiro[isobenzofuran-1(3h;5-(AND-6)-CARBOXYFLUORESCEIN;5(6)-FAM;5(6)-CARBOXYFLUORESCEIN;4(5)-CARBOXYFLUORESCEIN;3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid;5(6)-CARBOXYFLUORESCEIN, FOR FLUORESCENC E;5(6)-CARBOXYFLUORESCEIN 99% |
| CAS: | 72088-94-9 |
| MF: | C42H24O14 |
| MW: | 752.63 |
| EINECS: | 276-331-1 |
| Product Categories: | marker;Fluorescent Labels & Indicators;Electronic Chemicals;FLUOROPHORES;FAM |
| Mol File: | 72088-94-9.mol |
5(6)-Carboxyfluorescein Chemical Properties
| Melting point | 275 °C |
| Boiling point | 464.9°C (rough estimate) |
| density | 1.3418 (rough estimate) |
| refractive index | 1.6380 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | DMSO (Sparingly), Methanol (Slightly, Heated) |
| form | Solid |
| color | Light Yellow to Dark Brown |
| Water Solubility | slightly soluble |
| λmax | 492 nm |
| ε(extinction coefficient) | 12134 at 284nm in 0.1 M NaOH at 0.005g/L 43060 at 239nm in 0.1 M NaOH at 0.005g/L |
| Major Application | diagnostic assay manufacturing hematology histology |
| InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N |
| SMILES | C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9) |
Safety Information
| Hazard Codes | Xi,Xn |
| Risk Statements | 36-36/37/38-20/21/22 |
| Safety Statements | 37/39-26-36-22 |
| WGK Germany | 3 |
| TSCA | TSCA listed |
| HS Code | 29322090 |
| Storage Class | 11 - Combustible Solids |
| Description | 5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers. |
| Chemical Properties | Dark-Orange to Brown Solid |
| Uses | A popular probe of determination of intracellular pH. |
| Uses | 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. |
| Uses | As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent |
| Definition | ChEBI: 5(6)-carboxyfluorescein is a commercially available mixture of 5-carboxyfluorescein and 6-carboxyfluorescein isomers. A fluorescent dye, it is commonly used as a tracer agent. It has a role as a tracer and a fluorescent dye. It contains a 5-carboxyfluorescein and a 6-carboxyfluorescein. |
| reaction suitability | reagent type: fluorescent-labelling reagent |
| Solubility in organics | 5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively. |
5(6)-Carboxyfluorescein Preparation Products And Raw materials
